Table of Content

20 December 2013, Volume 13 Issue 06

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论文

SonoVue-enhanced ultrasound exposure (SEUE) improves the SPIO-labeling efficiency in Hep-G2 cells in vitro

Liang Huihong, Wan Wei, Shao Zili, Zhong Yun, Li Xi, Chen Fei, Fu Zhiqiang, Qian

2013, 13(06):  475-480.  DOI: j.issn.1009-976X.2013.06.002

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【Abstract】 Objective To investigate the feasibility of using SonoVue-enhanced ultrasound exposure (SEUE) to improve the efficiency of SPIO-labeling Hep-G2 cells in vitro. Methods Seven groups of SPIO at different concentration were incubated with Hep-G2 cells,.then processed by SonoVue-enhanced ultrasound exposure (SEUE) for 1 minute. The labeling efficiency, viability and proliferation were examined. The group of 375 μg[Fe]/mL SPIO-labeled Hep-G2 cells which achieved an appropriate level of proliferative activity at cellular densities (cells/mL) of 1×102, 1×103, 1×104, 1×105, 1×106, and 1×107 were processed by an in vitro MRI scan using a clinical 1.5T MRI system. Results A labeling efficiency >90% was achieved in seven experimental subgroups of group A, but they were about 2% in control group B and C. Hep-G2 cells was demonstrated reduced viability as the SPIO concentration increased. The viability of cells in four groups was only 59.7%±7% (500 μg[Fe]/mL), 47.76%±9% (625 μg[Fe]/mL), 46.27%±10% (750 μg[Fe]/mL), and 43.28%±7% (875 μg[Fe]/mL) respectively, which suggests that an SPIO concentration equal to or greater than 500 μg[Fe]/mL is obviously toxic to Hep-G2 cells. The proliferation of SPIO-labeled cells did not differ significantly. The in vitro MRI scanning data showed that the MRI signal intensity of labeled cells decreased with the T2W1 sequences which represents MRI can sensitively detect the SPIO concentration changes in cells. There was an inverse correlation between the labeling cell population and the signal intensity (P<0.05). Conclusions SonoVue-enhanced ultrasound exposure for improving the labeling of Hep-G2 cells with SPIO in vitro is feasible and effective method for MRI scanning.

The role of MEK/ERK1/2 in LPS-induced suppression of renal tubular epithelial NRK-52E cell proliferation

Lv Jun, Su Fang, Liang Weiwen, Liu Shanying

2013, 13(06):  481-484.  DOI: j.issn.1009-976X.2013.06.003

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【Abstract】 Objective To investigate the role of LPS on proliferation of renal tubular epithelial cells NRK-52E and the implication of MEK/ERK1/2 in the process. Methods NRK-52E cells were cultured and challenged with LPS at different concentrations and for the indicated time courses. A specific MEK inhibitor, U0126, was used either alone or as a pretreatment followed by adding LPS to the medium to test the involvement of MEK/ERK1/2 in the proliferation of NRK-52E cells exposed to LPS. Cell proliferation was evaluated using cell counting kit-8 (CCK-8) reagents. The protein levels of ERK1/2, Akt, phosphorylated ERK1/2 and Akt (pERK1/2, pAkt), were detected by Western blot using specific antibodies. Results LPS inhibited NRK-52E cell proliferation at the concentrations from 0.1 to 1.0 mg·L-1 after treatment for 72 hours. NRK-52E cell proliferation was also inhibited by U0126 at the concentrations from 2.5 to 20 μM after treatment for 72 hours,without significant difference between the tested concentrations. LPS-induced suppression of NRK-52E cell proliferation was further aggravated by pretreatment with U0126. LPS induced phosphorylation of both ERK1/2 and Akt, peaked at 30 min. LPS-induced phosphorylation of ERK1/2 was blunted by U0126 at 72 h, while phosphorylation of Akt was elevated in cells treated with U0126 alone as well as in combination with LPS. Conclusion LPS suppressed NRK-52E cell proliferation, in which MEK/ERK1/2 might play a protective role.

Comparison of additive EuroSCORE, logistic EuroSCORE, EuroSCOREⅡrisk models in application of evaluation of China cardiac surgical patients

Jiang Huiqi, Wang Meng, Zhang Shuming, Zeng Kuan, Hu Haiyu, Hua Ping, Yang Yanqi

2013, 13(06):  485-489.  DOI: j.issn.1009-976X.2013.06.004

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【Abstract】 Objective To evaluate the accuracy of additive EuroSCORE, logistic EuroSCORE and EuroSCOREⅡ in predicting the resident mortality of cardiac surgery. Methods Between January 2006 and October 2012, the clinical data of 594 patients in cardiothoracic surgery department, Sun Yat-sen Memorial Hospital, were retrospectively collected. The cohort was composed of 348 male and 246 female patients and the average age was 57.59±14.27 years. The expected mortality rate was calculated by additive EuroSCORE, logistic EuroSCORE and EuroSCOREⅡ retrospectively. According to the expected mortality, all the patients were divided into 6 groups (GroupⅠ0-1.99%, GroupⅡ2%-3.99%, GroupⅢ 4%-5.99%, GroupⅣ 6%-7.99%, GroupⅤ 8%-9.99%, GroupⅥ >10%). The observed and expected mortality of each group was compared. Calibration and discrimination were assessed using the Hosmer-Lemeshow (HL) Chi-square test and areas under the receiver operating characteristic (ROC) curve. Results The total observed mortality was 5.22% (31/594). The expected mortality was 3.46%±2.62% by additive EuroSCORE, 3.61%±4.32% by the logistic EuroSCORE and 2.81%±4.26% by EuroSCOREⅡ. EuroSCOREⅡ predicted the resident hospital mortality well except in group I and II in which the mortality was underestimated. EuroSCORE II had better discrimination than additive/logistic EuroSCORE (AUC 0.727 vs. 0.704, 0.715). P<0.05 for HL test of the logistic EuroSCORE while the other two systems had a good calibration. Conclusion EuroSCOREⅡ system is appropriate for predicting the mortality of patients in our heart center.

A comparative study of hand-assisted laparoscopic versus total laparoscopic splenectomy plus portal-azygous disconnection for portal hypertension in liver cirrhosis

Li En, Wu Zuguang, Liu Hongtao, Li Zhiwang, Chen Kai, Zhang Zhuoxin, etc.

2013, 13(06):  489-492.  DOI: j.issn.1009-976X.2013.06.005

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【Abstract】 Objective To explore the safety and feasibility of hand-assisted laparoscopic splenectomy plus portal-azygous disconnection for portal hypertension in liver cirrhosis. Methods From March 2008 to February 2013,.58 patients with portal hypertension were divided into two groups according to the patients' economic situation and wishes. HALS group was performed in 31 patients and were treated with hand-assisted laparoscopic operations, and TLS group in the other 27 patients were treated with total laparoscopic operations. The patient's operation time, intraoperative bleeding volume,.conversion to open surgery,.wound infection rate,.intestinal function recovery time, postoperative flatus time and hospital stay in both groups were compared..Chi-square test and t test was used. Results Fifty-eight patients were successfully operated..There were no significant differences in the aspects of intraoperative bleeding,.wound infection rate,.postoperative flatus time and hospital stay between the two groups(P>0.05). But the operation time and laparotomy rate were significantly shorter in HALS as compared TLS (P<0.05). Conclusion The operation of hand-assisted laparoscopic splenectomy plus portal-azygous disconnection would retain the minimally invasive advantages,.but also reduced the difficulty of surgery. It was safety and reliable, worthy of clinical application.

Analysis of conversion to open surgery in laparoscopic colorectal cancer surgery in 13 years

Cheng Longqing, Peng Xiang, Deng Jianzhong, Zhou Yonghui

2013, 13(06):  493-496.  DOI: j.issn.1009-976X.2013.06.006

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【Abstract】 Objective To evaluate the factors of conversion to open surgery in laparoscopic colorectal surgery, and choice the correct time turned to laparotomy during 13 years. Methods 1682 laparoscopic colorectal surgeries were included in the study. The reasons of conversion to open surgery from July 1999 to June 2012 were analyzed retrospectively. Results Among 1682 cases underwent laparoscopic colorectal surgeries, 1585 were performed successfully, 97 were turned to conversion to open surgery. The conversion rate was 5.77 %. The most common initiative reason was involvement of surrounding viscera or malignant tumor infiltration (53.8%, 49/91). The most common segment was sigmoid colon (24/91, 26.4%) and rectum (19/91, 20.9%). There were no statistical differences of operation time, intra-operative blood loss, recovery time of bowel movement, post-operative stay, lymph node yield and incidence of post-operative complications between the two groups. Conclusion In laparoscopic surgery for colorectal cancer, correct handling of indications for conversions to open surgery is an important step to ensure the safety of the patients.

Retroperitoneal laparoscopic ureteroureterostomy for retrocavalureter

Liu Hao,Dong Wen, Zhang Caixia, Bi Liangkuan, Yao Yousheng, Lin Tianxin,et. al.

2013, 13(06):  497-499.  DOI: j.issn.1009-976X.2013.06.007

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【Abstract】 Objective To report our experience with retroperitoneal laparoscopic ureteroureter-ostomy for retrocaval ureter. Methods From February 2008 to November 2012, 11 patients underwent retroperitoneal laparoscopic ureteroureterostomy for retrocaval ureter. Perioperative and Follow-up data were collected. Results All operations were completed unevenfully, without intraoperative caval injury and conversion to open surgery. The mean operating time was 119 minutes (range 85-160), the mean blood loss was 22 mL (range 10-40). Hydronephrosis were relieved substantially after surgery. Conclusion Retroperitoneal laparoscopic ureteroureterostomy for retrocaval ureter is a safe and feasible minimally invasive treatment option for a retrocaval ureter with satisfactory result.

Application of free-tandem flap in the repair of scalp defect with exposed cranium

Xu Lusheng, Guo Fan, Du Xueliang, Du Yongjun, Shen Rui, Li Jin, Xu Qikun

2013, 13(06):  500-502.  DOI: j.issn.1009-976X.2013.06.008

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【Abstract】 Objective To research the method of repairing large area scalp defect with exposed cranium. Methods Seven patients admitted from July 2005 to December 2012 were included in the study. Free-tandem flap comprising forearm flap and anterolateral thigh flap were applied in the repair of wide area scalp defect with exposed cranium in 7 patients. The proximal end of pedicle in forearm flap was anastomosed with superior thyroid artery and internal jugular vein. The distal end of pedicle in forearm flap was anastomosed with pedicle in anterolateral thigh flap. The non-skull-exposure area was repaired with skin grafting. Results All free-tandem flap survived well in 7 cases, with satisfactory appearance and texture. Conclusion Application of free-tandem flap comprising forearm flap and anterolateral thigh flap is an effective approach to repair wide area scalp defect with exposed cranium, and could achieve a good result.

Experience of Laparoscopic-assisted surgery for treating advanced colorectal cancer

Ye Yibiao, Luo Xingxi, Chen Jie, Zhang Jindai, Wu Xiao, Chen Tao

2013, 13(06):  503-506.  DOI: j.issn.1009-976X.2013.06.009

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【Abstract】 Objective To study the clinical efficacies on laparoscopic-assisted surgery in patients with advanced colorectal cancer. Methods The clinical data of 20 cases with advanced colorectal cancer in our hospital selected from January 2008 to July 2013 were retrospectively analyzed. All patients underwent laparoscopic-assisted surgery and the treatment-related evaluation indexes were recorded. Results Among that cases, 13 cases underwent simultaneous laparoscopy combined with open resection of the primary lesion and liver metastases, 2 cases underwent simultaneous laparoscopy combined with open resection of the primary lesion and abdominal lymph node metastases, 5 cases underwent laparoscopic palliative resection of the primary lesion. The average surgical time was 182±56 min, average blood loss was 340±28 ml, the first postoperative passing flatus time was 3.15±1.58 d,the hospitalization time was 10.26±1.89 d. There were four cases with postoperative complications, 2 cases of intestinal obstruction, 1 case of pulmonary infection, 1 case of electrolyte imbalance and all four patients were recovered under conservative treatment. Conclusion Laparoscopic-assisted surgery in patients with advanced colorectal cancer in technology is safe and effective. Palliative surgery will also contribute to reducing patient trauma, relieve the symptoms of intestinal obstruction and prolong life. Laparoscopic-assisted therapy would provide better options for some patients with advanced colorectal cancer.

Comparison of short-term curative effect between laparoscopic and open secondary hepatectomy in the treatment of liver metastasis of colorectal cancer

Long Xiaobin,.Guo Yi,Liu Xiaofei,Luo Jihui, Liu Hui,Deng Zhizhou

2013, 13(06):  507-510.  DOI: j.issn.1009-976X.2013.06.010

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【Abstract】 Objective To investigate the short-term effect between laparoscopic hepatectomy resection and open hepatectomy for liver metastasis of colorectal cancer. Methods From January 2008 to December 2012,.94 cases with liver metastasis of colorectal cancer underwent secondary hepatectomy,including laparoscopic operation in 46 cases,.open abdominal operation in 48 cases. Among 94 cases,.35 were underwent left hemihepatectomy,.28 right hemihepatectomy,.and 31 irregular wedge resection of liver. The resection of hepatic metastatic lesions were performed in three to six months after primary colorectal cancer resection. The short-term clinical efficacies were compared between two groups. Results Compared with open abdominal operation, laparoscopic hepatectomy had less intraoperative bleeding,.shorter hospitalization time and lower levels of postoperative alanine transaminase,.aspartate transaminase,.bilirubin and leukocyte count.(P<0.05). Conclusion For patients with hepatic metastases of colorectal cancer,.secondary hepatectomy laparoscopic operation is safe and reliable, and has better short-term effect than open opertion.

Experiences in management of pancreatic trauma (Report of 45 cases)

Huang Meitai, Chen Huanwei

2013, 13(06):  510-512.  DOI: j.issn.1009-976X.2013.06.011

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【Abstract】 Objective To summarize experiences in management of pancreatic trauma. Methods A total 45 cases with pancreatic trauma were included in the study. The data were collected from the patients admitted in The First People's Hospital of Foshan from 2003 to 2013 and were analyzed retrospectively..Results Among 45 cases,.12 underwent conservative treatment,.four were given ultrasound guided interventional therapy..Surgical procedure was performed in 29 cases,.including simple surgical drainage in six cases,.debridement and drainage in 11 cases,.partial pancreatectomy in four cases,.right hemihepatectomy+distal in one case,.pancreatectomy+splenectomy in one case, pancreaticogastrostomy in two cases,.Whipple procedure in four case,.combined repairment of liver in one case,.CBD,.duodenum and pancreatic injury+ gastrostomy and jejunostomy in one case..Forty-three patients were cured,.two patients died from multiple organs failure. Pancreatic fistula occurred in four cases and recovered by conservative treatment. Conclusion The individual treatment based on site and severity of pancreatic trauma is the key to improve cure rate.

Discussion on placing abdominal drainage-tube after cholecystectomy

Yang Yanqiong, Zi Xiangfan, Zhou Zhimei, Luo Tingguang

2013, 13(06):  513-514.  DOI: j.issn.1009-976X.2013.06.012

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【Abstract】 Objective To discuss whether to place the abdominal cavity drainage-tube after cholecystectomy and summarize the clinical experience. Methods Eighty-two patients admitted from April 2008 to April 2012 were included in the study. Of them,.53 cases weren't placed the abdominal cavity drainage-tube after cholecystectomy and 29 were placed in operation space. The operation time, hospital stay and complications were compared in two group of patients. Results All operations were completed..The patients with abdominal cavity drainage-tube had longer hospital stay and more complications than those without abdominal cavity drainage-tube. Conclusion Whether to place the abdominal cavity drainage-tube after cholecystectomy depends on the composite factors such as firm cystic duct ligation, thorough hemostasia, peritoneal contamination and surgical trauma.

Laparoscopic external drainage in the treatment of pancreatic pseudocysts associated with infection

Zhang Hongwei, Chen Yajin, Li Wenda

2013, 13(06):  515-517.  DOI: j.issn.1009-976X.2013.06.013

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【Abstract】 Objective To investigate the safety and feasibility of laparoscopic external drainage for management of pancreatic pseudocysts with infection. Methods In this retrospective analysis, 20 patients (15 men and 5 women) with infected pancreatic pseudocysts who underwent laparoscopic external drainage were reviewed. Results The mean operation time was 46.3 minutes and the hospital stay was 11.2 days. One post-operative patient suffered from abdominal infection due to pancreatic fluid leakage after operation. Pancreatic-abdominal fistula occurred in 3 patients and had pulmonary infection occurred in 2 cases. All patients recovered. The patients had to carry the drainage tubes for 36 d to 92 d (mean 45.5 d) after operation. Three patients had recurrence of pancreatic pseudocysts after pulling out the drainage tubes. Conclusion Laparoscopic external drainage is safe and feasible in the treatment of infected pancreatic pseudocysts.

The value of radial MR Imaging in menicus tear of the knee

Lei Lichang, Chen Jianyu, Yang Zehong, Cai Zhaoxi, Liu Zhenzhen, Li Yong

2013, 13(06):  517-520.  DOI: j.issn.1009-976X.2013.06.014

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【Abstract】 Objective To investigate the utility of the radial MR imaging in the interrogation of meniscal lesions and its instructional value for clinical management...Methods In the study,.we retrospectively evaluated 58 patients who underwent MR imaging of the knee and arthroscopy..The radial and conventional images were interpreted separately and then in combination,and findings were compared with arthroscopic reports. Results There was no statistical difference between the interpretations of the radial and conventional images. When the interpretations of the radial and conventional images were combined, the sensitivity and specificity improved slightly. Sensitivity and specificity for meniscal tears were accurate diagnosed by conventional scanning method (radial and conventional scanning method) were 62.50% (62.50%), 80.90% (95.23%), 72.72% (78.78%); 98.00% (94.00%), 89.19% (78.37%), 92.30% (92.30%). Sensitivity and specificity for meniscal tears were accurate diagnosed by conventional scanning method (radial and conventional scanning method) were 64.28% (78.57%), 76.47% (88.00%), 82.35% (94.11%), 97.72% (96.45%), 92.68% (85.36%), 97.56% (97.56%). Conclusion The radial images increased the conspicuity of meniscal tears. When the interpretations of the radial and conventional images are combined, the diagnostic accuracy increases, the radial MR Imaging is helpful means for the evaluation of meniscal tears.

Study of inhibition of dimethyl sulfoxide on morphine-induced thermal hyperalgesia

Du Sujuan,Yang Tao, Chen Ruixia, Ye Xijiu

2013, 13(06):  521-524.  DOI: j.issn.1009-976X.2013.06.015

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【Abstract】 Objective To explore the influence of dimethyl sulfoxide (DMSO) on morphine-induced thermal hyperalgesia and the expression of tumor necrosis factor-alpha (TNF-α) in the spinal cord dorsal horn. Methods Thirty-two SD rats were randomly divided into 4 groups, group A (saline + morphine group), group B (10% DMSO 5 mL/kg + morphine group), group C (10% DMSO 5 mL/kg + normal saline group) and group D (saline group). In each group, morphine or normal saline was injected subcutaneously 30 min after I.P normal saline or DMSO at 3 PM respectively. Paw withdrawal thermal latency (PWTL) were measured at 1 day before injection and 3, 6, 10 days after injection respectively and the expression of TNF alpha on spinal cord dorsal horn was detected. Results Compared with 1 day before injection, the PWTL of group A became shortened from 3 days after injection, and it further shortened at 10 day after injection respectively (P<0.01). There were significant differences in the shortening of PWTL between group A and group B 6 and 10 days after injection. The spinal dorsal horn TNF alpha expression of group A was higher than other groups (P<0.01). TNF alpha expression of group B were lower than which of group A and higher than which of group D. Conclusion DMSO can reduce morphine-induced thermal hyperalgesia by inhibiting spinal dorsal horn TNF alpha generation.

Impact of loading dose of dexmedetomidine on hemodynamic parameters monitored by Flotrac/Vigileo system in combined spinal-epidural anesthesia patients

Hu Chuwen, Chen Qianru, Wang Fei,.Zhao Yifan

2013, 13(06):  524-527.  DOI: j.issn.1009-976X.2013.06.016

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【Abstract】 Objective To observe the hemodynamic impact of loading dose of dexmedetomidine by Flotrac/Vigileo system monitoring. Methods Sixty patients scheduled for elective endourethral surgery under combined spinal-epidural anesthesia were randomized into two groups(group Dex and group Con). Group dex was infused dexmedetomidine as a loading dose of 0.5 μg/kg for 10min after spinal anesthesia blockage level stablized, while group Con received normal saline placebo infusion. The hemodynamic parameters were monitored by Flotrac/Vigileo system. Heart rate (HR), mean arterial pressure (MAP), cardiac output (CO), cardiac index (CI) and central venous pressure (CVP) were monitored. Systemic vascular resistance (SVR) was calculated in vigileo monitor and blood lactate was measure by blood gas analysis. Above parameters were recorded before anesthesia, after the level of anesthesia stable, and 1 min, 3 min, 5 min, 7 min, 10 min, 30 min after dexmedetomidine infusion. Results Compared with group Con, SVR decreased and CVP increased significantly 1 min after infusion in group Dex (P<0.05). CO and CI decreased 5min after infusion (P<0.05). MAP decreased 7 min after infusion (P<0.05). HR decreased 10 min after infusion (P<0.05). Conclusion Loading dose of dexmedetomidine slightly depressed MAP,.HR,.CO and SVR,.and increased CVP as well. Flotrac/Vigileo hemodynamic monitoring could reveal the hemodynamic changes earlier.

Value of MRI in the diagnosis of cerebral developmental venous anomaly

Xu Yuqin, Chen Yueping, Zhang Laishun, Jiang Yongjian, Tang Shuhui, Tang Huajuan

2013, 13(06):  528-530.  DOI: j.issn.1009-976X.2013.06.017

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【Abstract】 Objective To investigate the diagnostic value of MRI approaches in development venous anomaly.(DVA).Methods Sixteen patients with 17 DVA were included in this study and analyzed retrospectively. All cases underwent T1WI, T2WI, T2FLAIR, DWI plain and T1WI enhanced MRI scan,.and part of them underwent SWI and MRV scan. Results Among 17 DVA,.12 were located in supratentorial region and 5 in infratentorial region..Draining veins all showed flow void signal in T1WI,T2WI,T2FLAIR. Six cases of 16 cases were complicated with spongy angioma. Medullary veins showed strip or luffa cystic long T1, long T2 singal, low T2FLAIR signal. Draining and medullary veins all showed motting or strip low singal in DWI and SWI. Enhanced T1WI showed medullary veins drained into draining veins in spoke-wheel or arborization pattern. MRV showed intuitively the draining veins and the blood directions which presented like brain angiography,.but not as good as enhanced scan for medullary veins. Conclusion Combined with T1WI enhance and MRV,.MRI routine scan may confirm developmental venous anomaly and replace brian angiography.

Effect of ropivacaine combined with sufentanil on inhibiting the stress reaction for the colorectal carcinoma patients undergoing radical operation

Ma Zhijia,.Cheng Guorong

2013, 13(06):  531-533.  DOI: j.issn.1009-976X.2013.06.018

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【Abstract】 Objective To evaluate the effect of ropivacaine combined with sufentanil on inhibiting the stress reaction for the colorectal carcinoma patients undergoing radical operation. Methods Forty-six patients with colorectal carcinoma undergoing radical operation were randomly divided into the observation group (n=23) and the control group (n=23). The mixed liquor composed of 0.5 μg/mL sufentanil plus 0.15% ropivacaine was prepared for 15 mL. The patients in the observation group received injection of the mixed liquor from epidural space before skin incision,.while those in control group received the injection after recovering from an anaesthetic of surgery..The pain score (visual analogue scale,VAS).and the stress index were observed and analyzed in two groups. Results Compared with control group,.VAS in observation group was significantly lower than that in control group,.especially 3 h after surgery (P=0.026). The levels of white blood cells, neutrophils, alcohol, adrenocorticotropic hormone,..and c-reactive protein in observation group at 3 hour time point were significantly lower than that in control group(P<0.05). Conclusion Ropivacaine combined with sufentanil could effectively inhibit the stress reaction for the colorectal carcinoma patients undergoing radical operation.

Effect of anti-inflammatory-analgesic solution combined with low dose ozone injection by continuous infusion of epidural space on reliving pain for the patients with postherpetic neuralgia

Zhou Pu,.Yao Xinhua,.Wang Bao,.Qing Zhaohui

2013, 13(06):  534-536.  DOI: j.issn.1009-976X.2013.06.019

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【Abstract】 Objective To observe the curative effects of anti-inflammatory-analgesic solution combined with low dose ozone injection by continuous infusion of epidural space for the treatment of postherpetic neuralgia. Methods Sixty cases with postherpetic neuralgia were randomly divided into two groups(n=30 in each group). Group A:.epidural space infusion of anti-inflammatory-analgesic solution alone. Group B: epidural space infusion anti-inflammatory-analgesic solution combined with epidural catheter injection low dose ozone,.once a day..The changes of painful degree were observed during pre-treatment and different period of post-treatment in two groups..The therapeutic efficacies were evaluated by VAS score..Results In the VAS score,.the post-treatment was lower than that pre-treatment in two groups (P<0.05). Furthermore, since the second day of post-treatment, the every day VAS score of group B was lower than that of group A(P<0.05). Conclusion Continuous infusion anti-inflammatory-analgesic solution combined with low dose ozone injection in epidural space would promptly relieve pain and improve symptoms. It is an effective method for the treatment of postherpetic neuralgia.

Expression and significance of glutathione peroxidase 1 gene and super oxide dismutase 1 gene in adenoid cystic carcinoma

Liu Yeqing, Li Haifeng, Han Jingjing, Huang Zhiquan, Li Haigang

2013, 13(06):  537-540.  DOI: j.issn.1009-976X.2013.06.020

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【Abstract】 Objective To detect the expression of Glutathione Peroxidase 1 (GPX1) gene and Super Oxide Dismutase 1 (SOD1) gene in salivary adenoid cystic carcinoma (SACC) and demonstrate their potential correlation and theirs relationships with clinicopathological features and prognoses of SACC. Methods The expression of GPX1 and SOD1 in 35 SACC cases and 18 samples of adjacent salivary gland tissues were detected by immunochemical staining method. The potential correlation between GPX1 and SOD1 and clinicopathological features in SACC were analyzed. SPSS 13.0 software package was used for statistical analysis. Results The expression of GPX1 and SOD1 in the nucleus of SACC were significantly higher than that in adjacent salivary gland tissues (P<0.001). In SACC, the nuclear express of GPX1 and SOD1 were positively related with perineural invasion (Fisher's exact test, P=0.044 and 0.009). And the expression of GPX1 was positively related with the expression of SOD1(P=0.022). Conclusion The nuclear express of GPX1 and SOD1 were significantly correlated with the perineural invasion of SACC. It may serve as valuable markers for diagnosis and an indicator for prognosis of SACC.

Comparison of nerve growth factor and p75 neurotrophinc receptor expression in patients with degenerative disc diseasXe and lumbar disc herniation

Wang Gang, Mao RongJun, Wu Xunfu, Cao Zhenglin, Guang Honggang, Huo Zhiming

2013, 13(06):  541-544.  DOI: j.issn.1009-976X.2013.06.021

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【Abstract】 Objective To investigate the expression of nerve growth factor (NGF) and its receptor, P75 neurotrophin receptor (P75NTR) in disc specimens obtained from patients with lumbar disc herniation (LDH) and degenerative disc disease (DDD). Methods Human intervertebral disc samples were obtained from patients during surgery. The expression of NGF and P75NTR was detected by immunohistochemistry method. Results Immune positive staining was observed for both NGF and P75NTR..Cellular staining was predominantly localised intracellularly within the cytoplasm of chondrocyte-like cells of the nucleus pulposus and annulus fibrous tissue..NGF and P75NTR immunopositivity were 25.8% and 23.7% in disc tissue from LDH patient respectively..For DDD patient,the immunopositivity of NGF and P75NTR were 39.7% and 38.7% respectively.Immunopositivity of NGF and P75NTR were significantly greater in the DDD group than in the LDH group..No statistical correlation between the levels of NGF and P75NTR and the degree of disc degeneration in either DDD group or LDH group was found. Conclusion Our results demonstrate that the greater levels of NGF and P75NTR expression in the DDD group than in the LDH group may contribute more severe low back pain in DDD patients than patients with LDH.

Effect of ω-3 fish oil lipid emulsion on intestinal permeability and bacterial translocation in patients with intestinal obstruction

Li ying, Li Jinzhan, Wu Xueyan, Wu Junwei, Lu Bihui, Huang Zhuangsheng,

2013, 13(06):  545-548.  DOI: j.issn.1009-976X.2013.06.022

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【Abstract】 Objective To investigate the effect of ω-3 fish oil emulsion on intestinal permeability and bacterial translocation. Methods Ninety patients with intestinal obstruction were randomized into treatment group and controls. Controls received conventional treatment, including fasting, gastrointestinal decompression, correcting water electrolyte and acid-base balance disorders, parenteral nutrition and antibiotics. Treatment group used ω-3 fish oil emulsion on the basis of conventional therapy. Peripheral blood were collected for detection of routine serum biochemical, plasma glutamate (Gln) concentration and bacterial DNA. Meanwhile, urinary lactulose/mannitol (L/M) ratio was measured. The indexes above were compared between two groups. Results Serum albumin, serum prealbumin, serum transferrin in both groups increased higher significantly (P<0.05) while blood glucose was obviously lower (P<0.05). In the control group, IgA of the late course decreased sharply than the earlier(P<0.05), but there was no significant change in the treatment group. Plasma ω-3 fish oil emulsion concentrations in the treatment group was higher (P<0.01) than the control group,but the urinary lactulose/mannitol (L/M) ratio in the late course and the positive rate of bacterial DNA in peripheral blood were lower than that in control group (P<0.01). Conclusions ω-3 fish oil emulsion plays an important role in maintaining gut barrier function in patients with intestinal obstruction, at the same time, it can reduce bacterial translocation and complications.

Effect of ω-3 fish oil on nutrition and immune function after operation of malignant tumor of the gastrointestinal tract

Chi Liangjie, Huang Liangxiang, Zeng Changqing

2013, 13(06):  549-551.  DOI: j.issn.1009-976X.2013.06.023

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【Abstract】 Objective To study the effect of ω-3 fish oil on nutrition and immune function in postoperative patients with gastrointestinal malignant tumors. Methods Sixty cases of gastrointestinal malignant tumor from May 2010 to May 2013 were studied. All patients were randomly divided into experimental group and control group, with 30 cases of patients in each group. All patients were got parenteral nutrition support with equal nitrogen and calorie postoperatively, but the experimental group added ω-3 fish oil on the basis of routine treatment. All patients’ liver function, blood lipids, Hb, ALB, TF, lymphocyte count (TLC), B lymphocyte subsets (B1, B2), immunoglobulins (IgG, IgM, IgA) and complement(C3,C4) level preoperatively were measured in postoperative day 1 and postoperative day 6 respectively. Results There were no significant differences between experimental group and control group in liver function, blood lipid, but in postoperative day 6, the significant differences were found in the levels of TLC, IgG, IgM, C3 between the two groups(P<0.05). Conclusion Omega-3 fish oil has no significant effect on the postoperative nutrition for gastrointestinal cancer patients, while it would improve the immunity of the patients.

Comparative study on laparoscopic appendectomy and open appendectomy in children

Zhou Jiajia, Deng Xiaogeng, Zhang Jie, Wu Yaohao, Zeng Lexiang, Qiu Ronglin,

2013, 13(06):  552-555.  DOI: j.issn.1009-976X.2013.06.024

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【Abstract】 Objective To comparative study the clinical effect on laparoscopic appendectomy (LA) and traditional open appendectomy (OA) for treating appendicitis in children. Methods Ninety-three cases of children with appendicitis during hospitalization from 2009 January to 2012 December treated by LA or OA were analyzed retrospectively. The operation time, intraoperative blood loss and postoperative recovery situation of the LA and OA groups were compared. Results The surgical operation and postoperative recovery of children patients in both two groups were successful and no severe complications (except 6 cases of incision infection, one in LA, five in OA) occurred during and after operation. The operative time and blood loss of two groups were not significantly different (P>0.05). The passing flatus time, off-bed ambulation time, incision pain time, postoperative hospital stay of LA group were lower than that in OA group, and the difference had statistical significance (P<0.05). Conclusion Compared with open appendectomy, laparoscopic appendectomy in children has the advantages of small wound, less complications, faster recovery and nice wound healing, which could be an ideal treatment for pediatric appendicitis patients.

Clinical analysis of 7 case of biliary fistula after radical resection of gastric cancer

Li Dingyun, Zhang Tao, Lin Yafei, Lai Jiajun, Zeng Deqiang, Zhu Xiaofeng,

2013, 13(06):  555-557.  DOI: j.issn.1009-976X.2013.06.025

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【Abstract】 Objective To investigate the etiology, clinical manifestations, diagnosis and treatment of the biliary fistula after radical resection of gastric cancer. Methods The clinical data of seven patients with biliary fistula after radical gastrectomy were retrospectively analyzed. Results Two patients were found biliary fistula symptoms in the third day after surgery and two case appeared in the fourth day. The other trree patients were found biliary fistula in sixth, seventh and tenth, respectively day after surgery. All seven patients were appeared right upper quadrant pain in varying degrees, tachycardia (HR>120 bmp), fever (body temperature>38.5℃) and elevated blood leukocyte (WBC count>15×109/L). Six of 7 patients were cured by conservative treatment in three to eight weeks, and another one patients were cured after reoperation drainage of 8 weeks. Conclusion Intraoperative common bile duct injury is the main cause of biliary fistula after the radical resection of gastric cancer. Postoperative early diagnosis and adequate drainage is the key to cure the biliary fistula after radical resection of gastric cancer.

Clinical analysis of laparoscopic totally extraperitoneal patch implantation (TEP) for treating recurrent inguinal hernia in 33 cases

Tan Kanghui, Li Jun, Li Wei, Chen Zhiwei

2013, 13(06):  558-560.  DOI: j.issn.1009-976X.2013.06.026

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【Abstract】〓Objective〓To evaluate laparoscopic totally extraperitoneal patch implantation (TEP) for treating recurrent inguinal hernia and summarizing the experience. Methods From January 2010 to January 2013, clinical data of 33 cases undergoing TEP in our hospital were retrospectively analyzed and operation skills were summarized. Results All the operations were successfully completed, and no patients were transferred to open operation. Average operation time was (75.1±38.5) min, intraoperative blood loss was (9.5±3) mL, the average hospitalization time was (5.1±1.5) d. The complications had peritoneal laceration in 3 cases, subcutaneous emphysema in 1 case. No persistent postoperative neuropathic pain, groin hematoma, infection of incisional wound. All patients were followed up in a period of one to nine months. The recurrence occurred in one case 5 months after operation. Conclusion This surgical procedure is a safe and effective operative method in the treatment of recurrent inguinal hernia,and it would reduce significantly postoperative re-recurrent rate for recurrent hernia.

Research status and progresses in the patients satisfactory assessment after total joint arthroplasty

Zheng Shicong, Li Shiyuan, Zhang Yi

2013, 13(06):  561-566.  DOI: j.issn.1009-976X.2013.06.027

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【Abstract】 Objective To analyze the current status and progress of patient satisfaction assessment after total joint arthroplasty. Methods A search of recent articles of patient satisfaction assessment after total joint arthroplasty, and analysis on the validity, reliability, and responsiveness of the latest research progress were carried out. Results So far, there is still no gold standard in the patient satisfaction assessment after total joint arthroplasty. However, most research results tend to combine the specific questionnaires and generic questionnaires in the satisfaction assessment after total joint arthroplasty. Conclusion In recent years, more and more orthopedists are paying attention to the selection of appropriate patient satisfaction assessment methods after total joint arthroplasty. A comprehensive result can be obtained by the combination of specific questionnaires and generic questionnaires in patient satisfaction assessment after total joint arthroplasty.

Cause and treatment strategy of postoperative pain following hemorrhoidal operation

Zhong Xiongdong, Chu Zhonghua

2013, 13(06):  567-569.  DOI: j.issn.1009-976X.2013.06.028

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【Abstract】 Pain is the most common complication after haemorrhoids surgery, which obviously affects the therapeutic results and the patients' quality of life..Pain management after haemorroids surgery is also considered as an important issue in the anorectal surgery..This review is dedicated to explain the reasons and mechanisms of the pain after haemorrhoids surgery,.and the current strategies for it, which may provide some information for anorectal surgeons.

A case of strangulated small bowel obstruction and necrosis due to a paracecal hernia

2013, 13(06):  570-571.  DOI: j.issn.1009-976X.2013.06.029

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Effect on listening music therapy and breathing training for relieving adverse reactions for patients undergoing laparoscopic cholecystectomy

Deng Chunmei, Chen Xiaofeng

2013, 13(06):  571-573.  DOI: j.issn.1009-976X.2013.06.030

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【Abstract】 Objective To observe the effect of music therapy and breathing training on relieving adverse reactions for patients undergoing laparoscopic cholecystectomy. Method Eighty cases receiving laparoscopic cholecystectomy were randomly divided into interventional group (40 cases) and control group (40 cases) by random number table..The middle flow oxygen and early assisted ambulation were given as postoperative routine in control group,on the managed bases of control group,.the music therapy and breathing training were increased in the intervention group..The control group was flowed oxygen and assisted early ambulation in postoperative routine..Intervention group on the basis of control group received listening to music and breathing training. The patient's painful onset time, initial time and painful degree of postoperative 12 h,24 h and 48 h were assessed Visual Analogue Scale (VAS) and the incidences of adverse reaction were observed. Result In the patient's painful onset time, the interventional group was significantly delayed than that control group..In the initial pain and the painful degree of postoperative 12 h,24 h and 48 h,.the interventional group was reducer than that control group compared with the two groups, the difference was significant(P<0.001). Conclusion music therapy and breathing training would relieve postoperative pain and adverse reactions in the patients undergoing laparoscopic cholecystectomy under general anesthes and improve patient comfort.