Table of Content

20 December 2012, Volume 12 Issue 06

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Expression and clinical significance of Lnc RNA HOTAIR in esophageal squamous cell carcinoma

2012, 12(06):  407-409. 

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【Abstract】Purpose To explore the Expression and clinical significance of HOTAIR in the tissues of esophageal squamous cell carcinoma. Methods The expression of HOTAIR in 93 ESCC tissues、20 para - carcinoma tissues were detect by In situ hybridization. The relationship between the Expression of HOTAIR and clinicopathologic characteristics was analyze by spss16.0. Results In situ hybridization showed that the expression of HOTAIR in esophageal squamous cell carcinoma was higher than that in the tumor-adjacent non-malignant tissues. The expression of HOTAIR is related to T class、stages、lymph node metastasis、differentiation，(P＜0.05).but not related to age and gender of esophageal squamous cell carcinoma (P＞0.05).Conclusions HOTAIR could be used as a mark in occurrence and progress of ESCC.

Compariation of tissue explant and enzyme digestion method for rabbit bladder transitional epithelial cells in vitro

2012, 12(06):  410-412. 

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Objective Bladder transitional epithelial cells from rabbit were primary cultured by tissue explant and enzyme digestion method respectively to explore the better culture technique in vitro and provide seed cells for engineering of bladder tissue. Methods Rabbit bladder transitional epithelial cells were primary cultured by tissue explant and enzyme digestion method respectively. The cell morphology and proliferation characteristics were oberserved. The grow curve of the cells was described through cell counting.The cells were identified by cell morphology and immunofluorescence. Results The bladder transitional epithelial cells were obtained by the two methods possessed a typical morphological feature of epithelial cells. And the morphology and growth characteristics were essentially consistent. The growth curve of the cells was similar to “S”shape. It showed vimentin negative and cytokeratin AE1/AE3 positive. Conclusion Both the culture methods could obtain bladder transitional epithelial cells highly purity. The method of tissue explant has a higher success rate but spending more time than that of enzymatic digestion.

Imaging diagnosis of insulinoma

2012, 12(06):  415-417. 

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Objective To analyze the Multislice CT(MSCT) and MRI features of insulinoma. Methods MSCT and MRI findings in eighteen patients with surgical-pathologically-proved insulinoma were retrospectively analyzed. Results Seventeen cases of insulinoma had only one single tumor in every patient, except one case had three lesions. The range of the maximum diameter was between 0.3 and 2.0cm. Most of the insulinoma appeared iso-density and light hypo-density on CT plain scan. MRI showed hypo-intensity signal and light hypo-intensity signal on T1WI and hyper-intensity signal and light hyper-intensity signal on T2WI. These cases showed intensely enhancement on the arterial phase and iso-enhancement on the parenchymal and portal venous phases on dynamic contrast enhancement (DCE) MSCT and DCE MRI. Conclusion The insulinoma reveals some characteristic imaging features, which were based on the pathological changes. DCE MSCT should be the preferred means of screening insulinoma. The relevant ratio of the insulinoma can be significantly improved by rational utilization of DCE MSCT and MRI.

The clinical effects of extrahepatic lithiasis by laparoscopy combined with duodenoscopy.

2012, 12(06):  418-420. 

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Objective: To investigate the clinical effect of laparoscopic cholecystectomy ( LC) combind with endoscopic retrograde cholangiopancreatography(ERCP) and endoscopic sphincterotomy ( EST) in sequential treament of extrahepatic lithiasis．. Methods: From January 2006 to December 2009, The clinical records of 420 consecutive patients suffered from extrahepatic lithiasis were reviewed. The clinical effects were received by followed up. Result: 398 of 420(94.7%) consecutive patients who suffered from common bile duct stones were performed by ERCP+EST successfully. 71 patients had the early complications such as hyperamylasemia,pancreatitis,the bleeding of the duodenum papilla. No patient had the complication of the perforation of intestine. After bile duct stone clearance, except of 57 patients whom had been treated by cholecystectomy,185 of 363 patients underwent LC. 5 patients were found residual stones in the common bile duct by the imaging checking and were cleared by the second step of the ERCP+EST. Follow-up data were available in 311 patients with 6-54 months.During a median follow-up period of 32 months.The rate of follow-up is 74.0%.16(5.1%) patients were found that had the recurrent stones in the common bile duct. Conclusion: It is a safe and effective method for treating patients with extrahepatic lithiasis by EST sequential with LC．

Experience in dealing with bile duct injury due to laparoscopic cholecystectomy

2012, 12(06):  421-423. 

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Ultrasound characteristics of triple receptor-negative breast cancers.

2012, 12(06):  433-434. 

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Objective To compare ultrasound imaging features between triple-negative breast cancers and luminal breast cancers. Methods We analyzed ultrasound imaging features at initial diagnosis of 46 triple-negatibe cases and 260 luminal cases. Results Oval shape ( P= 0.000),circumscribed margin,( P=0.021 ) echo pattern（P=0.000）,posterior features（P=0.01） on sonogram were significantly associated with triple-negative breast .Microcalcifications was not（P=0.241）. Conclusions Triple-negative breast cancers had specific characteristic in sonogram .Sonography proves superior in detecting triple-negative breast cancers.

Clinical to explore and analysis of reserving intercostobrachial nerve during modified radical mastectomy

2012, 12(06):  435-437. 

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【Abstract】 Objective To explore the clinic value and application significance of modified radical mastectomy preserving intercostobrachial nerve. Methods To collect from February 2006 to February 2012 in our hospital to accept the surgical treatment of women with breast cancer 105 cases, the 32 patiens preserving intercostobrachial nerve analysis of the clinical data system review analysis, including 32 cases with intercostobrachial nerve preserved and 73 cases without preservation. Sensation function in media arms was observed . Results After 6 months to 3 years of follow up visit 32cases with intercostobrachial nerve preserved had normal sensation in media arms(87.5%), and 4 cases had abnormal sense (12.5%) , while all 73 cases without preservation had abnormal sensation (100%). Did not see local recurrence and metastasis. Conclusions Modified radical mastectomy preserving intercostobrachial nerve can effectively prevent sensation disturbance and decrease the incidence of complication. It has no effect on radical cure of surgery and can improve the qulity of patients' life.

Application of local anaesthesia in ultra-prolene hernia system inguinal hernia repair

2012, 12(06):  440-442. 

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[Abstract] Object To expore how to relieve the sufferings of patients during the operation of inguinal hernia repair using ultra-prolene hernia system under local anaesthesia and prevent postoperative pain in the inguinal region. Methods The mixed solution, consisted of 2% lidocaine (15mL) and 0.75% bupivacaine (5mL) or 1% ropivacaine (10mL), and diluted to 60 to 65 mL with saline, used as a local anaesthetic. Before separating preperitoneal space (Brogros space), 10 to 15 mL of the local anaesthetic was injected into the space for local infiltration (group B, n=77). Assessment of pain extent during suturing and fixing mesh was determined by numerical rating scale (NRS). 20 patients without undergoing injection of local anaesthetic were as controls (group A). Results All of controls (20/20,100%) felt apparent pain（NRS≥4）during separating preperitoneal space in group A, however only 6 cases in group B showed apparent pain (7.79%). The difference was significantbetween two groups (P<0.01). During suturing and fixing mesh, 18.56% patients (18/97) in group B felt pain of ligation, and the pain relieved promptly after removing the stitch causing pain. The operative time, length of hospital stay, blood loss, postoperative pain intensity and duration, complications, and recurrence rate between two groups had no significant differences (P>0.05). Conclusions The method that injecting the local anaesthetic into Brogros space for local infiltration during operation can alleviate the pain caused by performance of separating preperitoneal space. During inguinal hernia repair under local anaesthesia, informed the pain on patients when suturing and fixing mesh is helpful to prevent pain causing by ligation.

Clinical analysis of the diagnosis and operative treatment for scaphoid bone fracture and semilunal bone dislocation

2012, 12(06):  466-468. 

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Objective: To evaluate the diagnosis of scaphoid bone fracture and semilunal bone dislocation and the results of open reduction and internal fixation combined with intercarpal ligament repair for the treatment of this injury. Methods: form May 2005 to December 2011, 13 cases of scaphoid bone fracture and semilunal bone dislocation were treated operatively. There was a clear diagnosis from symptom and X ray and CT, All cases were treated with open reduction and internal fixation combined with intercarpal ligament repair.Results: All cases were followed up from 24 to 55 months, a mean period of 39.6 months. All the scaphoid bone healed primarily. According to clinical scoring of Cooney, the effective rate in postoperation is 84.6%.Conclusion: There was a clear diagnosis from symptom and X ray and CT, There was an effective method to cure this injury with open reduction and internal fixation combined with intercarpal ligament repair.

Advances of the treatment s to malignant bowel obstruction

2012, 12(06):  469-472. 

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Malignant bowel obstruction is a frequently complication caused by colorectal and gynecological malignancies, which can cause intestinal obstruction. Patients usually have a poor prognosis and their mean survival time is between 4-9months. The current treatments include: surgery、self-expanding stents、pharmacotherapy、percutaneous endoscopic gastrostomy and nasogastric tube. Besides, taking into account of the patients’ poor physical condition, parenteral alimentation can be used, however, there is still disputation on its efficacy. This passage will mainly focus on the current methods of MBO and make some analytic evaluation

内镜下治疗结肠息肉134例临床探讨

2012, 12(06):  477-478. 

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［目的］　：　探讨如何应用内镜下治疗摘除结肠息肉及降低并发症的发生率。［方法］：　使用OLYMPUS CF-Q260AI 电子结肠镜及WILSON WF-2421DQ 高频电凝器，OLYMPUS SD-221U-25 电圈套器进行结肠息肉摘除。［结果］：　摘除了126例共198颗结肠息肉，成功率100%。［结论］：严格遵守内镜下结肠息肉摘除操作规程和注意事项，可有较高治疗成功率并可减少并发症的发生。