Abstract: Objective To investigate the effect of adipose?derived stem cells （ADSCs） on the expanded flaps. Methods Expander model was established by using the adult female SD rats. Between skin and the expander, theAcellular dermis matrix（ADM）to which ADSCs adhere is held under skin. The expanded skin flaps were harvested after three weeks. Immunohistochemically stainingwas performed to characterize the form of organization.Western blot was performed to explore the change of Col Ⅰ and fibronectin protein expression. Transforming growth factor-β 1 （TGF-β 1） was measured by QPCR. Results The histologic sections demonstrated that ADSCs promote TGF-β1. On the first week and the second week postoperatively, the ColⅠand fibronectin significantly increased with ADSCs, but reduced remarkably in the third week. QPCR demonstrated high expression of TGF-β 1 in the first week after operation, then it declined with time. Conclusion ADM loaded with ADSCs provided a new experimen-tal basis to promote reconstruction of dermis of expander flap in expander operation.

Key words: adipose?derived stem cells, expander, acellular dermis matrix

摘要： 目的　观察脂肪干细胞对扩张皮瓣的影响。方法　将雌性SD大鼠制备成扩张器模型，皮肤下方与扩张器之间放置着附着大鼠脂肪干细胞（ADSCs）的脱细胞真皮基质（ADM）。培养 3周后获取扩张皮瓣，免疫组化分析组织形态变化，使用westernblot分析作用部位皮瓣内ColⅠ、ColⅢ和fibronectin蛋白表达情况，使用QPCR检测作用部位皮瓣内转化生长因子β1（TGF?β1）的表达量。结果　三周后组织切片提示ADSCs促进扩张皮瓣真皮内TGF?β1表达升高；术后第一周及第二周，在ADSCs的作用下，扩张皮瓣中ColⅠ和fibronectin蛋白表达明显上升，第三周有一个明显衰减，而ColⅢ表达量平稳，无明显变化趋势；QPCR提示术后第一周TGF?β1高表达，之后其表达量随时间衰减。结论　ADSCs诱导皮肤组织中的TGF?β1增加，进而促进真皮层下层成纤维细胞分泌更多的胶原结构与蛋白，促进扩张皮瓣真皮层修复。

关键词: 脱细胞真皮基质, 脂肪干细胞, 扩张器