Abstract:

【Abstract】 Objective To determine the effect of PIP controlled-release stents which specific silencing of TGF-β1 in prevention of ureteral stricture. Methods A rabbit model of ureteral stricture was constructed in 20 New Zealand rabbits and was randomzed into four groups, each group was 5 rabbits. Control group（group A）, general stent group（group B）, PIP targeting TGF-β1 controlled release stent （group C） and the mismatching PIP group （group D）. Animals were killed at 30 days and defined segments of ureteral were sectioned and observed about ureteral stricture degree, the expression level of TGF-β1 and a-SMA. Results There was different degree of stenosis in all four groups of ureteral lumen, however, group C was the lowest degree of stenosis（P<0.01）. The average thickness of narrow segment ureteral was 2.4±0.6 nm in group A, 2.1±0.7 nm in group B, 1.5±0.4 nm in group C and 2.2±0.4 nm in group D. Compared with the other groups, the expression levels of TGF-β1 and a-SMA were significantly lower in group C（P<0.01）. Conclusion PIP controlled-release stents which specific silencing of TGF-β1 can effectively reduce the degree of ureteral stricture occurred.

Key words: PIP, Ureteral stricture, TGF-β1

摘要： 【摘要】目的 确定特异性沉默TGF-β1的PIP控释支架在预防输尿管狭窄中的作用。方法20只健康纯种新西兰大耳白兔随机分为对照组（A组）、普通支架组（B组）、特异性沉默TGF-β1的PIP控释支架组（C组）和PIP错配组（D组），建立输尿管狭窄模型并置入相应的支架管，于术后30天取出损伤部位输尿管，对比不同组间输尿管狭窄的程度、TGF-β1和a-SMA的表达水平。结果4组输尿管管腔均出现不同程度的狭窄，其中C组狭窄程度最低（P<0.01）。狭窄段输尿管壁平均厚度分别为：A组2.4±0.6 nm，B组2.1±0.7 nm，C组1.5±0.4 nm，D组2.2±0.4 nm。与其它组相比，C组中TGF-β1和a-SMA表达水平均明显下调（P<0.01）。结论特异性沉默TGF-β1的PIP控释支架能有效减少输尿管狭窄发生的程度。

关键词: TGF-β1, 输尿管狭窄, PIP