Abstract: 【Abstract】〓Objective〓To explore the effects of Vps4A on Wnt signaling in hepatocellular carcinoma SMMC-7721 cells. Methods〓SMMC-7721 stable cell lines expressing Vps4A was generated. Then, the mRNA levels of total 19 Wnt subtypes were tested using RT-qPCR. The results were further confirmed by Western blot and investigating the downstream gene Notch2. Results〓Wnt10a and Wnt10b were highly expressed in SMMC-7721 cells, and Vps4A overexpressing significantly reduced their expression. Additionally, the levels of protein and mRNA of their downstream gene Notch2 were also obviously decreased. Conclusion〓Overexpressing Vps4A inhibits the canonical Wnt/Notch2 signaling in SMMC-7721 cell.

Key words: Vps4A, Hepatocellular carcinoma, Wnt, Notch2

摘要： 【摘要】 目的 探讨Vps4A对肝癌细胞SMMC-7721中Wnt途径的影响。方法〓构建过表达Vps4A的SMMC-7721细胞稳定株,通过RT-qPCR的方法检测19个WNT基因的转录水平的变化,进一步通过Western blot及检测相关下游基因Notch2的变化进行确定。结果〓在SMMC-7721中,经典Wnt亚型Wnt10a和Wnt10b的mRNA水平较高,而在过表达Vps4A之后,其蛋白及mRNA水平均显著下降。同时,在过表达Vps4A细胞株中,与经典Wnt通路相关的下游靶基因Notch2在转录及表达水平同样明显下调。结论〓Vps4A选择性抑制肝癌细胞SMMC-7721的经典Wnt/Notch2途径。

关键词: Vps4A, 肝癌, Wnt, Notch2