Abstract: 【Abstract】〓Objective〓To compare the difference of the epirubicin sensitivity of the breast cancer cells between three dimensional soft agar and chitosan scaffold models. Methods〓The growth curve of breast cancer cells separately cultured in three dimensional soft agar and chitosan scaffold models were recorded by cell counting and colony counting assay, then the epirubicin sensitivity of breast cancer cells were measured by MTT assay and colony counting assay, and the IC50s of these cells were calculated by IC50 calculating software and the difference between the IC50s was analyzed by t test. Results〓Both in three dimensional soft agar and chitosan scaffold models, the breast cancer MCF-7 and 231 cells could reach a good growth state; the IC50s of the breast cancer cells between three dimensional soft agar and chitosan scaffold models were not significantly different (P>0.05). Conclusion〓The chemosensitivity of breast cancer cells are not different in three dimensional soft agar and chitosan scaffold models, which indicate that the three dimensional growing environment doesn’t significantaly impact the the chemosensitivity of breast cancer cells.

Key words: Breast cancer, Soft agar model, Chitosan scaffold model, Chemosensitivity

摘要： 【摘要】 目的 比较三维软琼脂模型和三维壳聚糖支架模型中乳腺癌细胞对化疗药物表柔比星敏感性的差异。方法〓(1)分别采用细胞计数和集落计数的方法在三维软琼脂模型和三维壳聚糖支架模型中检测乳腺癌细胞MCF-7和MDA-MB-231细胞的生长曲线;(2)分别用四甲基偶氮唑哇盐法(MTT)和集落计数的方法检测三维软琼脂模型和三维壳聚糖支架模型中表柔比星对乳腺癌细胞MCF-7和MDA-MB-231细胞的细胞生长抑制率曲线和集落形成抑制率曲线;(3)通过IC50计算软件计算出相应乳腺癌细胞的IC50值,最后用t检验的统计学方法比较不同模型中乳腺癌细胞的IC50值是否有统计学差异。结果〓三维软琼脂模型和三维壳聚糖支架模型中的乳腺癌细胞MCF-7、231细胞均可达到指数生长期即均可达到良好的生长状态;三维软琼脂模型和三维壳聚糖支架模型中乳腺癌细胞的IC50值差异无统计学意义。结论〓三维软琼脂模型和三维壳聚糖支架模型中乳腺癌细胞对化疗药物的敏感性无明显差异,提示不同的三维生长环境对乳腺癌细胞的化疗药物敏感性没有显著影响。

关键词: 乳腺癌, 软琼脂模型, 壳聚糖支架模型, 化疗药物敏感性