Abstract: 【Abstract】 Objective To investigate the effect of MMP-9 in the process of mMICA shedding to sMICA in human colon cancer cells. Methods MMP-9 siRNA plasmid was transfected into HT-29 cells, the expressions of MMP-9 and MICA mRNA were detected through qPCR, he proteins of MMP-9, sMICA and mMICA were detected by ELISA. Results Transfection of MMP-9 siRNA into HT-29 resulted in a significant decrease in the levels of MMP-9 mRNA and protein, decreased the shedding of sMICA form mMICA, and upgraded the produce of protein of mMICA, however, it didn’t affect the expression of mRNA of MICA. Conclusion Inhibiting the expression of MMP-9 can decrease the porduction of sMICA as well as increase mMICA.

Key words: Colon cancer, MMP-9, sMICA

摘要： 【摘要】 目的 探讨金属基质蛋白酶9(MMP-9)在结肠癌细胞膜型MICA(mMICA)脱落为可溶型MICA(sMICA)过程中的作用。方法 HT-29细胞转染MMP-9 siRNA质粒沉默48小时后,检测MMP-9、MICA mRNA表达以及MMP-9、mMICA及sMICA蛋白生成情况。结果 MMP-9 siRNA质粒转染HT-29细胞后显著降低MMP-9 mRNA,不影响MICA mRNA;细胞膜表面mMICA蛋白显著增加同时sMICA蛋白生成明显减少。结论 抑制MMP-9表达,可减少结肠癌细胞mMICA蛋白降解,同时下调sMICA蛋白生成。

关键词: 结肠癌, MMP-9, sMICA