Expression of NEK7 in patients with primary hepatocellular carcinoma and its clinical significance

doi:10.3969/j.issn.1009-976X.2021.06.004

Abstract

Abstract: Objective To explore the relationship between the expression of NEK7 in clinical cases and the clinical biological characteristics and prognosis of liver cancer patients. Methods Based on the liver cancer mRNA data provided by the TCGA database, Cox regression and LASSO screening were used to select important prognostic variables, and Spearman correlation analysis between the expression of NEK7 and closely related molecules for the prognosis of liver cancer was performed. Immumohistochemical staining was used to test the differences in NEK7 expression in cancer tissues, adjacent tissues and non-tumor liver tissues of liver cancer specimens, and the relationship between their different expressions and the pathological characteristics of liver cancer patients was analyzed. We also explored the prognostic differences of patients with different expression levels of liver cancer. Results The expression of mRNA molecules CCDC21 and GTF3C2, which closely related to the prognosis of liver cancer, had a significant correlation with NEK7. Univariate analysis showed that the factors affecting the prognosis of liver cancer patients were mainly the degree of tumor differentiation, whether there were tumor thrombi and NEK7 expression. The expression of NEK7 in liver cancer tissues was significantly increased in cancer tissues, and its expression level and intensity were related to degree of liver cancer differentiation, Child stage and whether there are cancer emboli. In patients with liver cancer that overexpressed NEK7, their survival time was significantly reduced. Conclusion There is a significant correlation between NEK7 and mRNA molecules closely related to the prognosis of liver cancer. The prognostic factors of patients with liver cancer mainly include the degree of tumor differentiation, whether it is associated with tumor thrombus and the expression of NEK. NEK7 is highly expressed in liver cancer tissues, and its expression level and intensity are related to degree of liver cancer differentiation, Child stage and whether there are cancer emboli. Survival analysis shows that patients with positive expression of NEK7 have significantly shorter survival time.

Key words: primary hepatocellular carcinoma, NEK7, prognostic analysis

摘要： 目的探究肝癌患者NEK7表达情况与患者临床生物学特征及预后的关系。方法基于TCGA数据库提供的肝癌mRNA数据,以Cox回归及LASSO筛查选择重要的预后变量,Spearman相关性分析进行肝癌预后密切相关分子与NEK7表达的相关性分析。免疫组化检验肝癌标本癌组织、癌旁组织及非肿瘤肝脏组织中的NEK7表达的差异性,分析其不同表达情况与肝癌患者病理学特征之间的关系,并探究其不同表达水平肝癌患者预后差异性。结果与肝癌预后密切相关的mRNA分子CCDC21及GTF3C2与NEK7之间的表达具有显著的相关性,单因素分析表明影响肝癌患者预后的因素主要有肿瘤分化程度、是否合并癌栓及NEK7表达情况,相较于癌旁组织及正常肝脏组织,肝癌组织中NEK7表达显著升高,且其表达水平及强度与肝癌分化程度、Child分级及是否合并有癌栓相关,高表达NEK7的肝癌患者,其生存时间较低表达者显著缩短。结论肝癌患者预后因素主要有肿瘤分化程度、是否合并癌栓及NEK表达情况。NEK7在肝癌组织呈高表达状态,其表达水平及强度与肿瘤组织分化程度、Child分级及是否合并有癌栓相关,阳性表达NEK7肝癌患者生存时间显著缩短。

关键词: 原发性肝细胞肝癌, NEK7, 预后分析

CLC Number:

R735.7

SONG Yan-zhou, KANG Zhi-long, SU Yi-nan, ZHANG Yi-ming, ZHAO Xin, LI Zhi-wei, LI Wei. Expression of NEK7 in patients with primary hepatocellular carcinoma and its clinical significance[J]. Lingnan Modern Clinics In Surgery, 2021, 21(06): 608-613.

宋燕州, 康志龙, 苏一男, 张毅明, 赵新, 李志伟, 李伟. NEK7在原发性肝细胞肝癌患者中表达及其临床意义[J]. 岭南现代临床外科, 2021, 21(06): 608-613.

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