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Lingnan Modern Clinics In Surgery ›› 2021, Vol. 21 ›› Issue (03): 287-293.DOI: 10.3969/j.issn.1009-976X.2021.03.007

• Original Articles and Clinical Research • Previous Articles     Next Articles

Porous ferroferric oxide targeted delivery of nigericin to induce pyroptosis combined with photothermal therapy for triple-negative breast cancer treatment

JIANG Wei1,2, PAN Yue2, YAO He-rui1,2, HU Hai1,2   

  1. 1. Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China;
    2. Key Laboratory of Epidemiology and Gene Regulation of Malignant Tumors, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
  • Contact: YAO He-rui, yaoherui@mail.sysu.edu.cn; HU hai, huhai@mail.sysu.edu.cn

多孔四氧化三铁靶向递送尼日利亚菌素诱导细胞焦亡联合光热疗法用于三阴型乳腺癌治疗的研究

江伟1,2, 潘越2, 姚和瑞1,2,*, 胡海1,2,*   

  1. 1.中山大学孙逸仙纪念医院肿瘤内科,广州510120;
    2.中山大学孙逸仙纪念医院广东省恶性肿瘤表观遗传学与基因调控重点实验室,广州510120
  • 通讯作者: *姚和瑞,Email: yaoherui@mail.sysu.edu.cn
  • 基金资助:
    国家自然科学基金(81730077, 81572596, 81972471)

Abstract: Objective To investigate the therapeutic effect of nigericin loaded with porous Fe3O4 nanoparticles combined with photothermal therapy for triple-negative breast cancer. Methods characterize synthesized porous Fe3O4 nanoparticles and loaded with nigericin. The Fe3O4@nigericin nanoparticle with 200 μg/ml was prepared to induce 4T1 pyroptosis. The cell killing ability was detected by CCK8, and the pyroptosis effect was verified by Western blot. Results The synthesized Fe3O4 showed uniform morphology and good photothermal conversion performance. The combination of loading nigericin and photothermal treatment showed stronger cell killing ability (P<0.001) and better pyroptosis effect (P<0.01). Conclusion Porous Fe3O4 nanoparticles loaded with nigericin combining with photothermal therapy can produce very good therapeutic effect for triple-negative breast cancer by inducing pyroptosis, which is a potential new treatment method for triple-negative breast cancer.

Key words: porous Fe3O4 nanoparticles, pyroptosis, triple-negative breast cancer

摘要: 目的 探讨多孔四氧化三铁(Fe3O4)纳米粒子负载尼日利亚菌素(nigericin)联合光热疗法用于三阴型乳腺癌的治疗效果。方法 对合成的多孔Fe3O4纳米粒子进行表征,负载尼日利亚菌素,使用含有200 μg/mL的Fe3O4@nigericin诱导4T1细胞焦亡,通过CCK8检测细胞杀伤能力,western blot验证焦亡效果。结果 合成的Fe3O4形貌均一,光热转化性能好,负载尼日利亚菌素联合光热治疗具有更强的细胞杀伤能力(P<0.001),诱导的焦亡效果更好(P<0.001)。结论 多孔Fe3O4纳米粒子负载尼日利亚菌素联合光热疗法可以通过诱导焦亡对三阴型乳腺癌产生非常好的治疗效果,是三阴型乳腺癌的一种潜在的新型治疗手段。

关键词: 多孔四氧化三铁纳米粒子, 焦亡, 三阴型乳腺癌

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