Effect of overexpression of POLE2 on invasion and migration of non-small cell lung cancer cells

doi:10.3969/j.issn.1009-976X.2020.03.009

Abstract

Abstract: Objective To investigate the effect of POLE2 on the invasion and migration of non-small cell lung cancer （NSCLC） cells. Methods The expression of POLE2 in human NSCLC cells BEAS-2B and A549,human bronchial epithelial cell line SPC-A1 was detected by real-time PCR （qRT-PCR）. The POLE2-overexpression cell was established. The transfection efficiency was verified by qRT PCR and Western blot. Cell counting kit-8 （CCK-8） was used to detect the proliferation of NSCLC cells, Transwell was used to detect the invasion and migration of NSCLC cells, and qRT-PCR was used to detect the expression of MMP9. Results The expression level of POLE2 in human NSCLC cells SPC-A1 and A549 was significantly higher than that of BEAS-2B （P<0.05）. Overexpression of POLE2 promoted the proliferation, invasion and migration of A549 cells, and upregulated the expression of MMP9（P<0.05）. Conclusion Overexpression of POLE2 may promote the invasion and migration of NSCLC cells by up regulating MMP9.

Key words: migration, POLE2, invasion, non-small cell lung cancer

摘要： 目的探讨POLE2对非小细胞肺癌（NSCLC）细胞侵袭迁移能力的影响。方法采用实时荧光定量PCR（qRT-PCR）检测人支气管上皮样细胞BEAS-2B和NSCLC细胞A549、SPC-A1中POLE2的表达水平。构建POLE2过表达细胞模型,qRT-PCR和蛋白质印迹法（Western blot）验证转染效率。采用Cell Counting Kit-8（CCK-8）实验检测NSCLC细胞的增殖能力,Transwell实验检测NSCLC细胞的侵袭迁移能力,qRT-PCR检测MMP9的表达水平。结果人NSCLC细胞SPC-A1、A549中POLE2的表达水平显著高于人支气管上皮样细胞BEAS-2B（P<0.05）。过表达POLE2促进了A549细胞的增殖和侵袭迁移,上调了MMP9的表达（P<0.05）。结论过表达POLE2可能通过上调MMP9促进NSCLC细胞的侵袭迁移。

关键词: 迁移, 侵袭, NSCLC, POLE2

CLC Number:

R734.2

JI Lu-peng, PENG An-ping, ZHANG Yi, LIANG Ting-du. Effect of overexpression of POLE2 on invasion and migration of non-small cell lung cancer cells[J]. Lingnan Modern Clinics in Surgery, 2020, 20(03): 307-310.

姬路鹏, 彭安萍, 张毅, 梁庭都. POLE2对非小细胞肺癌细胞侵袭迁移能力的影响[J]. 岭南现代临床外科, 2020, 20(03): 307-310.

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