Abstract: Objective To investigate whether the healing speed of acute skin avulsion wound in mice was different between medical glue and traditional dressing change. Methods Eighteen C57 mice were randomly divided into three groups to make acute skin avulsionwounds, six mice in each group, namely medical glue group, vaseline group, and control group. The dressing were changed every 2 days for a total of 10 days. On the 0 th, 2 th, 6 th and 10 th days, the condition of wound healing was observed, the expressions of inflammatory mediators IL-6 and TNF-α were detected by Western blotting and ELISA respectively. On the 10 th day, the expression of IL-6 and TNF-α were observed by immunohistochemistry. Results On the 2 th, 6 th and 10 th days,the healing speed of the glue group was the fastest, followed by the control group, and the vaseline group was the slowest. For the expressions quantity of the IL-6 and TNF-α by Westernblotting and ELISA, the glue group was the lowest, followed by the control group, and the vaseline group was the highest. On the 10 th day,for the expression levels of IL-6 and TNF-α byimmunohistochemistry, the glue group was the lowest, followed by the control group, and the vaseline group was the highest. Conclusion Compared with traditional dressing change, medical glue can reduce inflammatory mediatorsand accelerate the healing of acute skin avulsion wound.

Key words: medical glue, IL-6, TNF-α, acute wound

摘要： 目的 探讨医用胶水的创面应用与传统换药方法在小鼠急性皮肤撕脱创面的修复愈合速度方面有无差异。方法 18只C57小鼠随机分为三组制作急性皮肤撕脱创面,每组各6只,分别为医用胶组、凡士林组、对照组,2天换一次药,共10天。分别于第0、2、6、10天大体观察创面愈合情况;免疫印迹（Western blotting）及酶联免疫（ELISA）检测炎症介质IL-6、TNF-α表达情况。第10天免疫组化观察IL-6、TNF-α表达情况。结果 第2、6、10天观察创面修复情况,医用胶组修复最快,其次为对照组,凡士林组修复最慢;免疫印迹及酶联免疫检测IL-6、TNF-α的表达情况,医用胶组表达最少,其次为对照组,凡士林组表达最多。第10天免疫组化观察IL-6、TNF-α表达量,医用胶组表达最少,其次为对照组,凡士林组表达最多。结论 与传统换药方法相比,医用胶水具有减少炎性介质、加快急性皮肤撕脱创面修复愈合的作用。

关键词: 医用胶, IL-6, TNF-α, 急性创面