Effect of Sanqidan granule on restenosis after balloon expansion of femoral artery in diabetic rabbit

doi:10.3969/j.issn.1009-976X.2020.06.010

Abstract

Abstract: Objective To investigate the effect of Sanqidan Granule on blood vessels after balloon dilatation of femoral artery in diabetic rabbits. Methods New Zealand white rabbits were randomly divided into blank group, control group and traditional Chinese medicine group; the blank group did not do any treatment and was fed with common feed; the control group and traditional Chinese medicine group were treated with alloxan intravenous injection to establish a diabetic rabbit model. after successful diabetes modeling, they were fed with high fat, and both groups received balloon dilatation femoral artery. The control group was fed with clear water and the traditional Chinese medicine group was fed with Sanqi Dan granule solution. fasting blood glucose （FBG）, total cholesterol （TC）, triglyceride （TG）, high-density lipoprotein （HDL-C）, low-density lipoprotein （LD） were then tested The contents of high sensitive C reactive protein （hs-CRP）, interleukin-6（IL-6）, tumor necrosis factor-a （TNF-a） and endothelin （ET） were determined by ELISA method. Results Sanqidan granule can improve the content of FBG, TC, TG, HDL-C, LDL-C, inhibit the content of hs-CRP, IL-6, TNF-α, ET and other inflammatory factors, pathological results suggest that the balloon dilatation caused by intimal proliferation and lumen stenosis. Conclusion Sanqidan granule can improve the vascular disease after balloon dilatation of femoral artery in diabetic rabbits.

Key words: diabetic rabbit, Traditional Chinese medicine therapy, Sanqidan granule, balloon dilation, restenosis

摘要： 目的探讨三芪丹颗粒对糖尿病兔股动脉球囊扩张后血管的影响。方法将兔随机分为空白组、对照组和中药组;空白组不做任何处理,并予普通饲料喂养;对照组和中药组采用四氧嘧啶静脉注射建立糖尿病兔模型,糖尿病建模成功后均予高脂喂养,两个组均接受了球囊扩张股动脉术。造模完成后对照组予清水喂食,中药组予三芪丹颗粒溶液喂食。术后4周处死,然后检测空腹血糖（FBG）、总胆固醇（TC）、甘油三脂（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）含有量,ELISA法测定血浆中高敏C反应蛋白（hs-CRP）、白细胞介素-6（IL-6）、肿瘤坏死因子-a（TNF-a）、内皮素（ET）含有量,同时行HE染色后观察血管狭窄情况,并行油红O染色观察血管脂肪含量。结果三芪丹颗粒可改善FBG、TC、TG、HDL-C、LDL-C 含有量,降低外周血hs-CRP、IL-6、TNF-a、ET等炎症因子水平;病理结果提示三芪丹可减轻因球囊扩张造成的内膜增殖和管腔狭窄。结论三芪丹颗粒可以改善糖尿病实验兔股动脉球囊扩张后的血管病变。

关键词: 球囊扩张, 中药疗法, 三芪丹颗粒, 糖尿病兔, 再狭窄

CLC Number:

R654.3

MO Wei, XU Xiu-ting, XIAO Zhi-xiong, ZHANG Wen-zhi, LIN Yi-xia, ZHOU Hui, WANG He. Effect of Sanqidan granule on restenosis after balloon expansion of femoral artery in diabetic rabbit[J]. Lingnan Modern Clinics in Surgery, 2020, 20(06): 732-735.

莫伟, 许秀婷, 肖志勋, 张文智, 林奕霞, 周晖, 汪何. 三芪丹对糖尿病兔股动脉狭窄的研究[J]. 岭南现代临床外科, 2020, 20(06): 732-735.

References

[1] 卞丽香, 宋开兰, 孙秋英, 等. 下肢血管介入对糖尿病下肢血管病变合并糖尿病足的临床价值及预后[J]. 医学影像学杂志, 2007(10):1089-1091.
[2] Faglia E, Mantero M, Caminiti M, et al.Extensive use of peripheral angioplasty, particularly infrapopliteal, in the treatment of ischaemic diabetic foot ulcers: clinical results of a multicentric study of 221 consecutive diabetic subjects[J]. J Intern Med, 2002,252(3):225-232.
[3] Morrish NJ, Wang SL, Stevens LK, et al.Mortality and causes of death in the WHO Multinational Study of Vascular Disease in Diabetes[J]. Diabetologia, 2001,44(Suppl 2):S14-S21.
[4] DeRubertis BG, Pierce M, Ryer EJ, et al. Reduced primary patency rate in diabetic patients after percutaneous intervention results from more frequent presentation with limb-threatening ischemia[J]. J Vasc Surg, 2008,47(1):101-108.
[5] Conte M S.Diabetic revascularization: endovascular versus open bypass--do we have the answer?[J]. Semin Vasc Surg, 2012,25(2):108-114.
[6] Agrawal NK, Maiti R, Dash D, et al.Cilostazol reduces inflammatory burden and oxidative stress in hypertensive type 2 diabetes mellitus patients[J]. Pharmacol Res, 2007,56(2):118-123.
[7] Shibata R, Kai H, Seki Y, et al.Role of Rho-associated kinase in neointima formation after vascular injury[J]. Circulation, 2001,103(2):284-289.
[8] 蒙绪标, 符兰芳, 刘婷婷, 等. 可溶性内皮细胞蛋白C受体和高敏C反应蛋白水平预测糖尿病患者血管并发症的价值[J]. 中国现代医学杂志, 2016,26(14):58-62.
[9] Kandhai-Ragunath J J, de Wagenaar B, Doelman C, et al. Endothelial function after ST-elevation myocardial infarction in patients with high levels of high-sensitivity CRP and Lp-PLA2: A substudy of the RESPONSE randomized trial[J]. Cardiovasc Revasc Med, 2017,18(3):202-206.
[10] Assoian R K, Marcantonio E E.The extracellular matrix as a cell cycle control element in atherosclerosis and restenosis[J]. J Clin Invest, 1997,100(11 Suppl):S15-S18.
[11] Negoro N, Hoshiga M, Seto M, et al.The kinase inhibitor fasudil (HA-1077) reduces intimal hyperplasia through inhibiting migration and enhancing cell loss of vascular smooth muscle cells[J]. Biochem Biophys Res Commun, 1999,262(1):211-215.
[12] Liang JJ, Xue W, Lou LZ, et al.Correlation of restenosis after rabbit carotid endarterectomy and inflammatory cytokines[J]. Asian Pac J Trop Med, 2014,7(3):231-236.