Effects of sevoflurane on right ventricular hypertrophy induced by monocrotaline in rats

doi:10.3969/j.issn.1009-976X.2020.04.003

Abstract

Abstract: Objective To observe the effects of sevoflurane on right ventricular hypertrophy（RVH） induced by monocrotaline in rats and explore its mechanisms. Methods Thirty male Spague-Dawlay rats were randomly assigned to a control or a monocrotaline group （60 mg·kg^-1）. After 4 weeks,rats were anesthetized,instrumented with an RV conductance catheter,and underwent dose-responses to sevoflurane inhalation （0.5%,1.0%,and 1.5%）. The changes of hemodynamics, right ventricular hypertrophy index （RVHI）, right ventricular myocardial infarction （RVMI）,inducible nitric oxide synthase （iNOS） and soluble guanylate cyclase （sGC） of right ventricular myocardium were analyzed. Results Compared with the control group, rats injected with monocrotaline presented elevated RVSP, RVHI and RVMI. The RVSP and MAP of all rats decreased after sevoflurane inhalation,and RVH rats preserved more dP/dtmax, cardiac output（CO）, stroke volume（SV）, and ejection fraction（EF）. The expression level of iNOS in right ventricle increased significantly while the level of sGC decreased obviously compared to the controls. Conclusion Sevoflurane could inhibit the right ventricular function in rats, but RVH rats were more tolerant to low-dose sevoflurane inhalation, which may be related to the up-regulation of the expression of iNOS and the decrease of sGC in right ventricular myocardium.

Key words: monocrotaline, sevoflurane, right Ventricular hypertrophy

摘要： 目的研究七氟烷对野百合碱诱导的大鼠右心室肥厚的影响并探讨其可能机制。方法 30只雄性SD大鼠随机分为野百合碱组（M组）及空白对照组（C组）,M组腹腔单次注射野百合碱60 mg·kg^-1,C组腹腔单次注射相同容积溶剂。饲养4周后,两组大鼠分别进行开胸手术,右心室置入PV导管,之后分别梯度吸入0.5%、1.0%、1.5%浓度的七氟烷,测定右心室收缩压（RVSP）等血流动力学指标及右心室肥厚指数（RVHI）、右心室质量指数（RVMI）。HE染色观察右室心肌组织病理变化;Western blot法观察右室心肌中诱导型一氧化氮合酶（iNOS）及可溶性鸟苷酸环化酶（sGC）的表达。结果与C组比较,M组大鼠RVSP、RVHI、RVMI明显增高,心室肉眼观可见右室游离壁明显增厚。吸入七氟烷后,两组大鼠RVSP、MAP均降低,但M组大鼠dP/dtmax、SV、CO及EF下降幅度较C组小。M组右室心肌组织中iNOS表达高于C组,而sGC表达低于C组。结论低浓度七氟烷可抑制SD大鼠的右心功能,但右室肥厚大鼠更能耐受其抑制作用,其机制可能与右室心肌中iNOS表达增加及sGC表达减少有关。

关键词: 右心室肥厚, 野百合碱, 七氟烷

CLC Number:

R614

YIN Xiao-qing, ZOU Jun, WANG E. Effects of sevoflurane on right ventricular hypertrophy induced by monocrotaline in rats[J]. Lingnan Modern Clinics in Surgery, 2020, 20(05): 557-561.

尹小清, 邹军, 王锷. 七氟烷对野百合碱诱导的右心室肥厚的影响[J]. 岭南现代临床外科, 2020, 20(05): 557-561.

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