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Lingnan Modern Clinics in Surgery ›› 2020, Vol. 20 ›› Issue (03): 307-310.DOI: 10.3969/j.issn.1009-976X.2020.03.009

• Original Articles and Clinical Research • Previous Articles     Next Articles

Effect of overexpression of POLE2 on invasion and migration of non-small cell lung cancer cells

JI Lu-peng1, PENG An-ping2, ZHANG Yi3, LIANG Ting-du1   

  1. 1. Department of Medicine, The Fifth People's Hospital of Zhuhai, Guangdong 519055, China;
    2. South Campus Clinic, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China;
    3. Department of Respiratory Medicine, Zhuhai People's Hospital, Zhuhai, Guangdong 519000, China
  • Contact: ZHANG Yi, 231917657@qq.com

POLE2对非小细胞肺癌细胞侵袭迁移能力的影响

姬路鹏1, 彭安萍2, 张毅3,*, 梁庭都1   

  1. 1.珠海市第五人民医院内科,广东珠海519055;
    2.中山大学孙逸仙纪念医院南校园门诊部,广东广州510120;
    3.珠海市人民医院呼吸科,广东珠海519000
  • 通讯作者: *张毅,Email: 231917657@qq.com
  • 基金资助:
    珠海市卫计局医学科研基金(2016-25)

Abstract: Objective To investigate the effect of POLE2 on the invasion and migration of non-small cell lung cancer (NSCLC) cells. Methods The expression of POLE2 in human NSCLC cells BEAS-2B and A549,human bronchial epithelial cell line SPC-A1 was detected by real-time PCR (qRT-PCR). The POLE2-overexpression cell was established. The transfection efficiency was verified by qRT PCR and Western blot. Cell counting kit-8 (CCK-8) was used to detect the proliferation of NSCLC cells, Transwell was used to detect the invasion and migration of NSCLC cells, and qRT-PCR was used to detect the expression of MMP9. Results The expression level of POLE2 in human NSCLC cells SPC-A1 and A549 was significantly higher than that of BEAS-2B (P<0.05). Overexpression of POLE2 promoted the proliferation, invasion and migration of A549 cells, and upregulated the expression of MMP9(P<0.05). Conclusion Overexpression of POLE2 may promote the invasion and migration of NSCLC cells by up regulating MMP9.

Key words: migration, POLE2, invasion, non-small cell lung cancer

摘要: 目的 探讨POLE2对非小细胞肺癌(NSCLC)细胞侵袭迁移能力的影响。方法 采用实时荧光定量PCR(qRT-PCR)检测人支气管上皮样细胞BEAS-2B和NSCLC细胞A549、SPC-A1中POLE2的表达水平。构建POLE2过表达细胞模型,qRT-PCR和蛋白质印迹法(Western blot)验证转染效率。采用Cell Counting Kit-8(CCK-8)实验检测NSCLC细胞的增殖能力,Transwell实验检测NSCLC细胞的侵袭迁移能力,qRT-PCR检测MMP9的表达水平。结果 人NSCLC细胞SPC-A1、A549中POLE2的表达水平显著高于人支气管上皮样细胞BEAS-2B(P<0.05)。过表达POLE2促进了A549细胞的增殖和侵袭迁移,上调了MMP9的表达(P<0.05)。结论 过表达POLE2可能通过上调MMP9促进NSCLC细胞的侵袭迁移。

关键词: 迁移, 侵袭, NSCLC, POLE2

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