Abstract: Objective To observe correlation between thermal response latency and sciatic nerve myelin associated protein in streptozotocin?induced mouse with diabetes. Methods Sixteen 6?week?old male SPF grade C57BL/6J mice were randomly divided into two groups with 8 mice each. STZ 200mg/kg in STZ group was injected intraperitoneally once to establish the mouse model with diabetes. The same volume of buffer was injected in CON group. Mice were placed on a hot plate apparatus to value the thermal response latency. Real time RT qPCR and western blot were used to detect the expression of MAG，P0 in the sciatic nerve of mice. The correlation between the thermal response latency and MAG、 P0 was analyzed. Results Compared with CON group，the FBG level of STZ group was significantly increased（P<0.0001），which showed that the STZ induced diabetes model was successfully established. Compared with CON group，the thermal response latency of STZ group was longer（P<0.05）. The mRNA and protein expression levels of MAG、P0 were all significantly decreased in group A than those in group B（P<0.0001）. Conclusion The sciatic nerve myelin associated proteins are attenuated in the STZ?induced mouse model with diabetes. The results suggest that peripheral hyperglycemia may cause thermalgesia，which might relative to the decrease of myelin associated protein. But the specific mechanism re? mains to be explored.

Key words: Mouse diabetes model, Streptozotocin, Myelin associated protein, Thermal response latency

摘要： 目的 观察链脲佐菌素（streptozotocin，STZ）诱导的糖尿病模型小鼠热痛反应潜伏时间与坐骨神经髓鞘相关蛋白表达的相关性。方法 16 只6 周龄雄性SPF 级C57BL/6J 小鼠随机分成两组，每组 8 只。STZ 处理组小鼠采用一次性腹腔注射STZ 200 mg/kg，建立小鼠糖尿病模型；对照组注射同等体积缓冲液。采用 YLS?6A 智能热板仪进行热痛反应潜伏期测量，记录各组小鼠热痛反应潜伏期。采用实时定量PCR 检测两组小鼠坐骨神经髓鞘相关蛋白MAG、P0 mRNA 的表达；采用 western blot 检测髓鞘相关蛋白 MAG、P0 的蛋白表达水平。并分析小鼠热痛反应潜伏时间与髓鞘相关蛋白表达水平的相关性。结果 与对照组比较，STZ 处理组小鼠 FBG 升高（P<0.0001），成功建立糖尿病模型。与对照组相比，STZ 处理组小鼠热痛反应潜伏期延长（P<0.05），STZ 组小鼠热痛不敏感。与对照组比较，STZ 处理组小鼠坐骨神经髓鞘相关蛋白MAG、P0 mRNA（P<0.0001）以及蛋白水平的表达下降。结论 STZ 诱导的糖尿病模型小鼠热痛反应时间延长，与坐骨神经髓鞘相关蛋白表达水平下降相关。但具体机制仍待进一步探讨。

关键词: 髓鞘相关蛋白, 糖尿病模型小鼠, 链脲佐菌素, 热痛反应潜伏期