Abstract: ［Abstract］ Objective To observe the influence of morphine on proliferation and vascular endothelial growth factor （VEGF） expression of triple-negative breast cancer cell （MDA-MB-231 breast cancer cell）, and to explore the effect of PI3K?AKT?c?Myc signal pathway on this process. Methods MDA?MB? 231 breast cancer cells were divided into negative control group（N group）, 1 μmol/L morphine group （1 μM group）, 10 μmol/L morphine group（10 μM group）and 100 μmol/L morphine group（100 μM group）randomly. The cells in N group were incubated with normal cultural medium without FPS , 1μM group were incubated with 1 μmol/L morphine, 10 μM group were incubated with 10 μmol/L morphine and 100 μM group were incubated with 100 μmol/L morphine. After the treatments, MTS was applied to detect the proliferation of MDA?MB?231 breast cancer cells, while the expression of vascular endothelial growth factor（VEGF）, c?Myc and the phosphorylation of AKT were detected by Western Blot. Results Compared with the N group, each concentration of morphine in our experiment could promote the prolifer? ation of MDA?MB?231 cells, increase the expression of VEGF, c?Myc and the phosphorylation of AKT. The 10 μM group increases most significant（P<0.05）. Conclusion Morphine in the concentration of 10 μmol/L could increase MDA?MB?231 breast cancer cell proliferation and VEGF expression, activation of the PI3K?AKT?c?Myc signal pathway may related to this process.

Key words: proliferation, morphine, triple-negative breast cancer, angiogenesis, PI3K-AKT-c-Myc signal pathway

摘要： ［摘要］ 目的 探究吗啡对三阴性乳腺癌细胞（人乳腺癌MDA-MB-231 细胞）增殖及血管内皮生长因子（VEGF）表达的影响，并对 PI3K-AKT-c-Myc 信号通路的激活在此过程中的作用进行初步探讨。方法 将人乳腺癌MDA-MB-231 细胞随机分为阴性对照组（N 组）、1 μmol/L 吗啡组（1 μM 组）、10 μmol/L 吗啡组（10 μM 组）以及 100 μmol/L 组（100 μM 组），分别予无血清培养基、含1 μmol/L 吗啡的无血清培养基、含10 μmol/L 吗啡的无血清培养基及含100 μmol/L 吗啡的无血清培养基处理。处理后对各组细胞行MTS 法检测细胞增殖情况，并行Western Blot 实验检测细胞内VEGF、c-Myc 的表达及 AKT 蛋白磷酸化水平。结果 与N 组相比，不同浓度吗啡处理对人乳腺癌MDA-MB-231 细胞增殖、细胞内 VEGF、c-Myc 的表达及 AKT 蛋白磷酸化均有促进作用，其中以10 μM 组作用最为显著（P<0.05）。结论 10 μmol/L 吗啡可促进人乳腺癌MDA-MB-231 细胞增殖及VEGF 的表达，此过程可能与激活细胞内PI3K-AKT-c-Myc 信号通路有关。

关键词: 三阴性乳腺癌, PI3K-AKT-c-Myc 信号通路, 增殖, 吗啡, 血管新生