Abstract: 【Abstract】 Objective〓To explore the effect of combined argon-helium cryoablation and GM-CSF treatment on cytokine secretion of splenic DCs and CTLs cytotoxic activity in glioma-bearing mice...Methods〓Glioma-bearing mice models were established and divided into four groups of no-treatment,.GM-CSF injection,.cryoablation treatment,.and GM-CSF and cryoablation combined treatment. After seven days' treatment, the mice spleens were obtained, then the DCs were purified and cultured in vitro. The secretions of IL-10, IL-12p70 and IFN-γ were detected by ELISA. And the CD8+ CTLs were separated and cultured with GL261 cells in vitro..Then the cytotoxic activity of CD8+ CTLs were detected by LDH test. Results〓Combined treatment with cryoablation and GM-CSF could not only significantly decrease the secretions of IL-10, but also increase the secretions of IL-12 and IFN-γ..Furthermore,.combined treatment could signifcantly increase the cytotoxic activity of CD8+ CTLs..Conclusion〓Argon-helium cryoablation combined with GM-CSF treatment can further effectively activate T cells and enhance specific anti-tumor immunity through the stimulation of DCs and secretion of Th1 type cytokines.

Key words: Argon-helium cryoablation, Cytokine, Glioma, Dendritic cells

摘要： 【摘要】 目的〓探讨氩氦冷冻联合GM-CSF治疗对荷胶质瘤小鼠脾脏DCs分泌细胞因子及CTLs对肿瘤细胞杀伤活性的影响。方法〓建立C57BL/6小鼠皮下GL261胶质瘤模型,然后随机分为模型对照组、单纯GM-CSF注射组、单纯氩氦冷冻组和联合治疗组。治疗后7天取各组小鼠脾脏制备细胞悬液,部分体外培养,ELISA法检测上清IL-10、IL-12p70、IFN-γ的分泌情况;BD磁珠分选系统分离CD8+ CTLs,与GL261胶质瘤细胞共培养,LDH法检测脾脏CD8+ CTLs杀伤活性。结果〓单纯氩氦冷冻组和联合治疗组小鼠脾脏IL-10分泌水平较模型对照组下降,联合治疗组降低更明显(P均<0.05);单纯氩氦冷冻组和联合治疗组小鼠脾脏IL-12、IFN-γ分泌水平、脾脏CD8+ CTLs杀伤活性均较模型对照组升高,且以联合治疗组升高最为明显(P均<0.05)。结论〓氩氦冷冻联合GM-CSF治疗能通过刺激DCs,分泌大量Th1型细胞因子,从而进一步有效激活T细胞,增强机体特异性抗肿瘤免疫。

关键词: 胶质瘤, 树突状细胞, 氩氦冷冻治疗, 细胞因子