Abstract: 【Abstract】〓Objective〓To observe the therapeutic efficacy of carbon nanoparticle loaded with 5-fluorouracil in treatment of breast cancer model with metastasized axillary lymph nodes. To probe into the efficacy of carbon nanoparticles using in chemotherapy of breast cancer as a drug carrier. Methods〓Thirty female New Zealand white rabbits were randomly and equally divided into three groups, text group, Ⅳ(intravenous) group, HI group, with 10 in each. VX2 tumor mass suspensions (1 ml) was injected into the mammary gland (Hypodermic) of each animal..Treatment was given while axillary lymph node enlarged and reached 5 mm in the maximum diameter..Animals in test group and HI group received a subcutaneous injection of 5-FU-carbon nanoparticle suspension and 5-FU solution, respectively, adjacent to the implanted tumor. The IV group received an auricular intravenous injection of 5-FU solution..The dose of 5-FU in each group was 25 mg/kg..Animals were killed after the treatment was given 5 days. Growth rate of tumor and lymph node was measured. Necrosis degree and apoptotic index of tumor and lymph node after treatment were observed and measured. Results〓The growth rate of test group was lower than the other groups,.while necrosis degree and apoptotic index were higher than the other groups. There was statistical significance difference between these three groups (P<0.05). Conclusion〓Carbon nanoparticle could improve the treatment efficacy of chemotherapy drug in axillary metastatic lymph node of breast cancer.

Key words: Carbon nanoparticle, Drug carrier, Breast cancer, Chemotherapy

摘要： 【摘要】 目的 观察纳米碳吸附5-氟尿嘧啶对新西兰兔乳腺癌模型的治疗效果,探讨纳米碳作为药物载体在乳腺癌化疗中的应用效果。方法〓30只雌性新西兰兔分3组,实验组(CN)、静脉组(IV)和皮下组(HI),每组10只。动物通过肿瘤组织块悬液注射法建立模型,当腋窝触及直径5 mm大小肿大淋巴结时开始治疗。实验组皮下注射纳米碳-氟尿嘧啶混悬液;静脉组通过耳缘静脉注射5-氟尿嘧啶;皮下组在皮下注射5-氟尿嘧啶,药物剂量均为25 mg/kg。治疗5天后处死各组动物,测量并计算治疗前后瘤体及淋巴结增长率,HE染色评价组织中肿瘤细胞坏死程度,TUNEL检测肿瘤细胞凋亡指数。结果〓治疗5天后三组瘤体及淋巴结均有增长,病理切片可见肿瘤细胞坏死及凋亡,与其余两组相比,实验组淋巴结增长率较低,转移灶中肿瘤细胞坏死程度及凋亡指数较高,差异具有统计学意义(P<0.05)。结论〓纳米碳可能提高化疗药物对乳腺癌淋巴结转移灶的治疗效果。

关键词: 纳米碳, 载体, 乳腺癌, 化疗