Abstract: 【Abstract】〓Objectives〓To investigate the expression and clinical significance of miR-150 in hepatocellular carcinoma(HCC). Methods〓miR-150 expression in liver cancer and paired normal tumor-adjacent tissues was determined by quantitative Real-time PCR (qRT-PCR). The expression difference between HCC and tumor-adjacent tissues was further verified using in situ hybrization(ISH) technique. Association of miR-150 expression in HCC tissues with clinicopathological status was examined by correlation analysis. Prognostic significance of miR-150 expression in HCC samples was detected with Kaplan-Meier method and Cox regression analysis. Results〓qRT-PCR assay showed 8/12 HCC cases had lower miR-150 expression compared with tumor-adjacent tissues and miR-150 expression level in HCC tissues markedly decreased (P=0.034). Similar to qRT-PCR results, ISH technique demonstrated most HCC specimens had negative or weak staining of miR-150, and staining intensity in 62% tumor-adjacent tissues was much higher than that in paired HCC tissues (P<0.001). Correlation analysis suggested miR-150 expression was significantly negatively correlated with AFP level, tumor size, TNM stage, histological grade and intravascular thrombus (P<0.05). Postoperative overall survival (OS) of miR-150 positive group was much longer that of miR-150negative group (P=0.004, Kaplan-Meier method). Cox univariate regression analysis implicated low miR-150 level in HCC samples was an unfavorable prognostic indicator (P=0.005). Conclusion〓Low expression of miR-150 in HCC specimens is related with a more aggressive tumor phenotype and indicates poor prognosis for HCC patients.

Key words: MicroRNA-150, Hepatocellular carcinoma, Prognosis

摘要： 【摘要】〓目的〓探讨微小RNA(miR)-150在肝细胞癌中的表达及其临床意义。方法〓实时荧光定量聚合酶链反应(qRT-PCR)检测肝癌及配对癌旁组织中miR-150表达。原位杂交技术(ISH)进一步验证肝癌和配对癌旁组织miR-150表达差异。将肝癌组织中miR-150表达与临床病理特征进行关联分析。Kaplan-Meier法检测miR-150阴性组和阳性组生存曲线。Cox单因素回归模型分析miR-150表达与患者术后总生存的相关性。结果〓qRT-PCR结果显示:与癌旁组织相比,12例中有8例肝癌组织低表达miR-150,肝癌组织中miR-150表达明显降低(P=0.034)。ISH结果与qRT-PCR结果相一致,即肝癌组织大部分为阴性或弱阳性表达,62%癌旁组织ISH染色强度高于配对的肝癌组织(P<0.001)。关联分析提示miR-150表达与AFP、肿瘤大小、TNM分期、组织学分级、脉管癌栓存在明显负相关(P<0.05)。miR-150阳性组患者术后总生存明显高于miR-150阴性组(P=0.004,Kaplan-Meier法)。Cox单因素回归分析提示肝癌组织低表达miR-150是患者不良预后指标(P=0.005)。结论〓肝癌组织低表达miR-150与肿瘤侵袭表型相关,提示不良预后。

关键词: 微小RNA-150, 肝细胞癌, 预后