Abstract: 【Abstract】〓Objective〓To investigate the role of miRNA-144 in invasive hepatocellular carcinoma. Methods〓52 cases of HCC tissues and corresponding adjacent cancer tissue were collected from the Guangdong Provincial People?蒺s Hospital of HCC tissue bank. miRNA-144 expression was detected by in situ hybridization in hepatocellular carcinoma and corresponding adjacent cancer tissues. The relationship between miR-144 expression level and clinical pathology was characterized. Online software was used to predict the potential target genes of miR-144. Results〓miR-144 was relatively highly expressed in invasive HCC comparing to adjacent cancer tissue. miR-144 was positively associated with liver tumor grade and early recurrence. Online software predicted SRF as one of the candidate target gene of miR-144. Conclusion〓miRNA profiles in hepatocellular carcinoma may be associated with tumor grade and early recurrence and could serve as potential prognostic marker.

Key words: miR-144, Hepatocellular carcinoma, Invasion, SRF

摘要： 【摘要】〓目的〓探讨miRNA-144对肝癌细胞侵袭性的影响。方法〓从广东省人民医院肝癌标本库中选取52例肝癌组织标本及其对应的癌旁组织,用原位杂交技术检测miRNA-144在肝癌及相应的癌旁组织的表达情况,分析其与临床病理特征的关系,并行在线靶基因软件预测到miR-144的靶基因中有SRF。结果〓miR-144在肝癌组织中相对癌旁组织高表达,并且miR-144在肝癌组织中表达量仅与肝癌病理分级和术后早期复发有关,三大在线靶基因软件预测到miR-144的靶基因中都有SRF。结论〓筛选得到的肝癌早期复发的miRNA差异表达谱可能与肝癌的病理分级和早期复发有关。

关键词: miR-144, 肝癌, 侵袭, SRF