Abstract: 【Abstract】〓Objective〓To investigate the expression of CD4+CD25+ regulatory T cells (Treg) and its correlation with Hunt?蒺s classification for the patients with subarachnoid hemorrhage caused by intracranial aneurysm. Methods〓Fifty aneurysm patients with SAH,.50 cases with intracranial infection patients and 50 healthy people were included in this study. The peripheral blood examples were collected to detect the levels of transforming growth factors beta 1 (TGF-beta 1) and interleukin 10 (IL-10) by using flow cytometry. Levels of Tregs, TGF-beta 1 and IL-10 were compared between three groups,.and the correlation between Treg,.TGF-beta 1,.IL-10 and aneurysmal SAH Hunt classification were analyzed. Results〓The percentage composition of Treg, mean fluorescence intensity of TGF-beta 1 and IL-10 in patients with intracranial aneurysms group were significantly lower than those in healthy group,.and significantly lower than in the intracranial infection patients,.but no statistical difference was found between intracranial infection group and control group..Any of three levels of Treg,.TGF-beta 1 and IL-10 in aneurysm patients showed a negative correlation with any level of Ⅰ-Ⅴ Hunt classification(r absolute value is greater than 0.7, P<0.05),.in which the maximum correlation showed in Ⅴ level (P<0.001). Conclusion〓Treg, TGF-beta 1 and IL-10 could be protective factors of patients with intracranial aneurysm,.and lower expressions of the three factors would worsen the SAH condition.

Key words: Intracranial aneurysm, Subarachnoid hemorrhage, CD4+CD25+ regulatory T cells, Cytokines

摘要： 【摘要】〓目的〓探讨颅内动脉瘤致蛛网膜下腔出血(SAH)患者CD4+CD25+调节性T细胞(Treg)的表达及其与分级的关系。方法〓收集动脉瘤致SAH患者、颅内感染患者及健康人各50例,采用流式细胞术检测外周血Treg百分含量及其细胞因子转化生长因子(TGF-β1)及白介素10(IL-10)的平均荧光强度。比较三组上述三者的差异,并分析三者与动脉瘤致SAH的Hunt分级的相关性。结果〓动脉瘤组患者Treg、TGF-β1及IL-10均显著低于健康组,且明显低于颅内感染组,差异均有统计学意义,但颅内感染组与对照组无统计学差异。动脉瘤组患者Treg、TGF-β1及IL-10三者当中任意一者与Ⅰ~Ⅴ级当中任意一级均呈负相关性(所得r绝对值均大于0.7,P均小于0.05),三者均与Ⅴ级的相关度最大(P均小于0.001)。结论〓Treg、TGF-β1及IL-10可能是颅内动脉瘤患者的一个保护因素,其含量越低则破裂后SAH程度越重,因此三者具有重要临床意义。

关键词: 动脉瘤, 蛛网膜下腔出血, CD4+CD25+调节性T细胞, 细胞因子