Abstract:

【Abstract】〓Objective〓To explore the role of dendritic cells (DC)-derived dexosome (DEX) in proliferation regulation of human gastric adenocarcinoma cells after treated with hyperthermic CO₂. Methods〓Based on an in vitro hyperthermic CO₂ pneumoperitoneum experimental model,the DC-derived DEX was isolated and identified through TEM after treatment by hyperthermic CO₂. AGS cells were co-cultured with DEX in vitro. And the cell proliferation was detected by CCK-8 assay,the expression of p-AKT and bcl-2 in AGS cells was examined by Western blot, and relative activity of p-AKT and bcl-2 was determined by colorimetric assay. Results〓DEX was successfully isolated. The proliferation rate of AGS cells was 54.73%±2.04% in hyperthermic CO₂ treated group, lower than that of heat treated group and control group (P<0.05). Significantly lower expression of p-AKT and bcl-2 in AGS cells treated with hyperthermic CO₂-DEX was observed by Western blot. And the relative activity of p-AKT and bcl-2 in AGS cells treated with hyperthermic CO₂-DEX were 41.28%±2.31% and 36.49%±1.92%. Conclusions〓DC-derived DEX treated by hyperthermic CO₂ could significantly inhibit proliferation of AGS cells, which may be related to the inhibition of AKT pathway activation and the decrease of expression of bcl-2.

Key words: Hyperthermic CO2, Dendritic cell, Gastric cancer, Proliferation inhibition

摘要：

【摘要】〓目的〓探讨热CO₂处理后树突状细胞(DC)源性外泌体(DEX)在胃癌细胞增殖调控中的作用机制。方法〓建立热CO₂气腹体外实验模型,分组处理DC2.4细胞后,提取DEX并通过透射电镜鉴定其形态。体外培养胃癌AGS细胞,用各组DEX干预后以细胞计数试剂盒(CCK-8)法检测AGS细胞的增殖情况,蛋白免疫印迹法(Western blot)检测AGS细胞中蛋白激酶B(AKT)磷酸化水平和B细胞淋巴瘤-2(bcl-2)的表达,比色法检测p-AKT和bcl-2的活性。结果〓成功提取DEX,热CO₂气腹组AGS细胞的增殖率为54.73±2.04%,与单纯热处理组及对照组比较差异有显著性(P<0.05)。Western blot结果显示,热CO₂气腹组AGS细胞中p-AKT和bcl-2蛋白表达均显著降低。与对照组比较,热CO₂气腹组p-AKT和bcl-2相对活性为41.28±2.31%和36.49±1.92%。结论〓热CO₂处理DC后,DEX能显著抑制胃癌AGS细胞的增殖,其作用机制可能与抑制AKT通路活化和bcl-2表达及活性的降低有关。

关键词: 热二氧化碳, 树突状细胞, 胃癌, 增殖抑制