Mechanism of morphine on proliferation of pheochromocytoma cells

doi:10.3969/j.issn.1009-976X.2014.02.006

Abstract

Abstract: 【Abstract】〓Objective〓To investigate the effects of morphine on roliferation of pheochromocytoma cell and its mechanism. Methods〓PC12 cell cultures were exposed to 10,20,30 μmol/L morphine. The inhibition of the cell proliferation was determined by MTT assay. The level of p38MAPK was detected by immunoblotting. Results〓The proliferation of PC12 cells were inhibited after morphine exposure for 48 h.Immunoblotting showed that morphine increased the phosphorylation of p38MAPK was increased in stimulation of monphine. SB203580, the inhibitor of p38MAPK, reduced inhibition the proliferation of PC12 cells by morphine. Conclusion〓These results suggest that morphine can significantly inhibits the proliferation of PC12 cells by an increase in p38MAPK phosphorylation.

Key words: Morphine, PC12 cells, Proliferation, p38MAPK

摘要： 【摘要】〓目的〓探讨吗啡对大鼠嗜铬细胞瘤PC12细胞增殖的抑制作用及其分子机制。方法〓PC12细胞体外培养,将吗啡加入细胞培养液中使吗啡浓度分别稀释至10、20、30 μmol/L,然后分别与PC12细胞一同孵育,四甲基偶氮噻唑蓝法(MTT)观察吗啡对细胞增殖的抑制作用;免疫印迹法检测PC12细胞p38MAPK表达及磷酸化水平。结果〓不同浓度吗啡对PC12细胞增殖有明显的抑制作用,抑制率为17.66%~31.05%;并且吗啡可以诱导PC12细胞p38MAPK磷酸化水平升高,而p38MAPK通路阻断剂SB203580可以抑制吗啡诱导PC12细胞p38MAPK磷酸化水平升高,减轻吗啡对PC12细胞增殖的抑制。结论〓吗啡可以明显抑制PC12细胞的增殖,升高p38MAPK的磷酸化水平可能是其重要分子机制之一。

关键词: 吗啡, PC12细胞, 增殖抑制, p38MAPK

CLC Number:

R614

Chen Weiqiang, Liu Funing, Liu Ling, He Huiyan, Cao Minghui. Mechanism of morphine on proliferation of pheochromocytoma cells[J]. Lingnan Modern Clinics in Surgery, 2014, 14(02): 136-139.

陈伟强刘付宁刘玲何惠燕曹铭辉. 吗啡对PC12细胞增殖的抑制作用及机制初探[J]. 岭南现代临床外科, 2014, 14(02): 136-139.

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