Abstract: Objective To investigate the expression and significance of NF-кB and MUC1 in hepatolithiasis associated with intrahepatic cholangiocarcinoma. Methods Ninety cases of liver tissues specimen of hepatectomy were selected in The Second Affiliated Hospital of Sun Yat-sen University in recent 20 years. Among them, 33 cases with hepatolithiasis associated with intrahepatic cholangiocarcinoma were as experiment group, 32 cases with hepatolithiasis were as control group and 25 cases with normal intrahepatic duct tissues were an blank group. The immunohistochemical staining of NF-кB and MUC1 were performed by tissues slice.Results 1)There was significant difference in NF-кB and MUC1 positive expression rate (P＜0.05).2)MUC1 positive expression rate showed significant difference in histopathologic grading and in lymph node metastasis (P＜0.01,0.05).3)Accumulated survival rate of MUC1 expressed tumor patients was lower than that of MUC1 non-expressed tumor patients(P＜0.01). Conclusion NF-кB expression is probably the early stage in the carcinogenesis of intrahepatic cholangiocarcinoma and in the formation of hepatolithiasis. High level expression of MUC1 is related with malignan degree of cholangiocarcinoma and with worse prognosis.

摘要： 目的：探讨NF-кB、MUC1在肝内胆管结石合并肝内胆管癌组织的表达和意义。方法：选取中山大学附属第二医院近20年手术切除肝组织标本共90例,其中肝内胆管结石合并肝内胆管癌33例为实验组,肝内胆管结石32例为对照组,正常肝内胆管组织25例为空白组。组织切片进行NF-кB、MUC1免疫组化染色。结果: ①NF-кB、MUC1阳性表达率的差异均有统计学意义（P＜0.05）。②MUC1阳性表达率在肿瘤病理组织学分级、淋巴结转移的差异有统计学意义（P＜0.01,0.05）。③MUC1表达与否和肿瘤病人的累积生存率有关（P＜0.01）。结论：NF-кB的异常表达是肝内胆管结石及肝内胆管癌的早期事件，MUC1过表达与肿瘤恶性程度和预后有关。

关键词: NF-кB, MUC1, 胆管结石, 胆管癌, NF-кB, MUC1, lithiasis, cholangiocarcinoma