circZBTB44 promotes the proliferation and migration in clear cell renal cell carcinoma via regulating AKT signaling pathway

doi:10.3969/j.issn.1009-976X.2021.03.010

Abstract

Abstract: Objective To explore the expression of circZBTB44 in clear cell renal cell carcinoma （ccRCC） and investigate its biological effects and the possible mechanism. Methods Quantitative real-time PCR （qRT-PCR） were conducted to detect the expression of circZBTB44 in ccRCC tissues and cell lines, 786-O and ACHN. Actinomycin D assay, RNase R assay and RNA Nuclear and Cytoplasmic Extraction assay were applied to identify the characteristics of circZBTB44 in 786-O and ACHN. MTS cell proliferation assay, colony formation assay, Transwell assay were performed to investigate the biological effects of circZBTB44 in 786-O and ACHN cells after silencing circZBTB44. Western blotting analysis were employed to identify the downstream signaling pathways that might be modulated by circZBTB44. Results circZBTB44 was overexpressed in ccRCC tissues and cell lines. Silencing circZBTB44 suppresses the proliferation and migration capability of 786-O and ACHN cells. Downregulating the expression of circZBTB44 could inhibit the phosphorylation of AKT. Conclusion circZBTB44 was overexpressed in ccRCC and promoted the proliferation and migration ability of ccRCC via regulating AKT signaling pathway.

Key words: circZBTB44, AKT signaling pathway, clear cell renal cell carcinoma （ccRCC）, proliferation, migration

摘要： 目的探究circZBTB44在肾透明细胞癌中的表达情况及探讨circZBTB44在肾透明细胞癌中的生物学功能及促进肾透明细胞癌细胞进展的可能机制。方法通过qRT-PCR法检测circZBTB44在我院21对肾透明细胞癌组织及细胞系786-O、ACHN中的表达水平;通过放线菌素D实验、RNase R实验、核浆分离实验鉴定circZBTB44的特征;通过siRNA下调circZBTB44的表达后,通过MTS细胞增殖实验、克隆形成实验及Transwell迁移实验来探究circZBTB44对肾透明细胞癌786-O、ACHN的生物学功能影响;通过蛋白免疫印迹实验来探究circZBTB44调控的下游通路。结果 circZBTB44在肾透明细胞癌组织和细胞株786-O和ACHN中明显高表达。下调circZBTB44的表达可明显抑制786-O和ACHN的增殖和迁移能力。下调circZBTB44可抑制肾透明细胞癌786-O和ACHN细胞中AKT的磷酸化。结论 circZBTB44在肾透明细胞癌中高表达。circZBTB44可能通过调控AKT信号通路促进肾透明细胞癌的增殖和迁移。

关键词: circZBTB44, AKT通路, 肾透明细胞癌, 增殖, 迁移

CLC Number:

R737.11

ZHONG Qi-yu, XIE Wen-lian. circZBTB44 promotes the proliferation and migration in clear cell renal cell carcinoma via regulating AKT signaling pathway[J]. Lingnan Modern Clinics In Surgery, 2021, 21(03): 302-308.

钟启宇, 谢文练. circZBTB44通过调控AKT通路促进肾透明细胞癌增殖和迁移的研究[J]. 岭南现代临床外科, 2021, 21(03): 302-308.

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