Mesoporous silica nanoparticle-loading 5-Fluorouracil for potent antitumor effect against hepatic cancer and breast cancer cells

doi:10.3969/j.issn.1009-976X.2021.03.009

Abstract

Abstract: Objective To improve the therapeutic effect of anti-tumor drugs and effectively reduce the toxic side effects. Methods We synthesized mesoporous silica nanoparticles （MSN） loaded with the anti-tumor drug fluorouracil （5-FU） using the sol-gel method to obtain MSN@5-FU drug-loaded nanomaterials. The physical properties of MSN@5FU were characterized, next the drug loading, biocompatibility and drug release of MSN@5-FU were investigated, and finally the in vitro cancer cell killing effect was studied. Results The electron micrographs clearly observed that the synthesized MSN was nanoscale in size, with uniform size and obvious pore size distribution. Meanwhile, the synthesized MSN has a strong ability to load 5-FU. In addition, the drug loading system can exist stably in solution for a long time without toxic side effects on normal cells. Importantly, a large amount of 5-FU can be released in a micro-acidic environment. the results of live-dead cell experiments indicate that the nano-loaded system has good ability to kill cancer cells. Conclusion The MSN@5-FU nanodrug delivery system can play an important role in the treatment of hepatic and breast cancer.

Key words: mesoporous silica nanoparticles, loading capacity, cell viability

摘要： 目的为了提高抗肿瘤药物的治疗效果,有效降低毒副作用。方法利用溶胶-凝胶法合成介孔硅纳米颗粒（MSN）,负载抗癌药物氟尿嘧啶（5-FU）,得到MSN@5-FU载药纳米材料。首先对MSN@5FU的物理特性进行表征,接下来考察MSN@5FU的载药量,生物相容性以及药物释放情况,最后研究体外杀死肿瘤细胞的效果。结果电镜图可以清晰地观察到,合成的MSN是纳米级尺寸,大小均一,孔径分布明显。同时,合成的MSN具有很强的负载5-FU的能力。此外,该载药系统在溶液中可以长期稳定存在,对正常细胞无毒副作用。重要的是,在微酸环境下可以大量释放5-FU。活死细胞实验结果说明该纳米载药系统具有良好的杀死肿瘤细胞能力。结论 MSN@5FU纳米载药系统在肝癌和乳腺癌治疗中可以发挥重要作用。

关键词: 介孔硅纳米颗粒, 载药量, 细胞活性

CLC Number:

R737.9

SONG Yang, ZHONG Ke-li. Mesoporous silica nanoparticle-loading 5-Fluorouracil for potent antitumor effect against hepatic cancer and breast cancer cells[J]. Lingnan Modern Clinics In Surgery, 2021, 21(03): 298-301.

宋洋, 钟克力. 介孔硅纳米颗粒负载氟尿嘧啶用于肝癌和乳腺癌治疗的研究[J]. 岭南现代临床外科, 2021, 21(03): 298-301.

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