Lidocaine suppresses thyroid cancer cell TPC-1 proliferation via inhibiting activation of ERK signaling pathway

doi:10.3969/j.issn.1009-976X.2021.03.008

Abstract

Abstract: Objective To investigate the effects of lidocaine on thyroid cancer cell and its mechanism. Methods After treating thyroid cancer cell TPC-1 with 10 μM or 100 μM lidocaine in vitro, the changes of cell proliferation was detected by conducting CCK-8 assay, cell cycle was detected with flow cytometry and cyclin expression was determinged byWestern Blot. In vivo, after subcutaneous xenograft models established in BALB/c nude mice, comparison of the effect of lidocaine on tumor growth was performed between BALB/c nude mice andcontrols. Results After TPC-1 cells treated with 10 μM or 100 μM lidocaine in vitro, CCK-8 assay showed TPC-1 cell proliferation can be suppressed with 100 μM exerting stronger effect than 10 μM. Flow cytometry showed lidocaine-treated cells were attenuated in phase S while accentuated in phase G0/G1. Western blot showed p21 and p27 expressionswere both up-regulated while ERK and p-ERK expressions were both down-regulated in cells treated with lidocaine. Subcutaneous xenograft also showed tumor growth can be suppressed by lidocaine injection in nude mice. Conclusion Lidocaine can suppress proliferation of thyroid cancer cell TPC-1 both in vitro and in vivo. The mechanism could be inhibition of ERK signaling pathway activation.

Key words: thyroid cancer, Lidocaine, proliferation

摘要： 目的研究利多卡因对甲状腺癌细胞的影响及其作用机制。方法 10 μM、100 μM利多卡因处理甲状腺癌细胞TPC-1后,进行CCK8实验检测细胞增殖,流式细胞学实验检测细胞周期,Western Blot检测细胞周期蛋白变化。BALB/C裸鼠皮下成瘤实验比较利多卡因与对照组对肿瘤生长的影响。结果 10 μM、100 μM利多卡因处理后,CCK8结果显示两种浓度下TPC-1细胞增殖均可被抑制,且100 μM利多卡因抑制作用强于10 μM。流式细胞学结果显示两种浓度下均有S期细胞比值降低,G0/G1期细胞比值增加。Western Blot结果显示两种浓度下均有p21、p27蛋白表达量升高,ERK与p-ERK表达下调。皮下成瘤实验结果显示利多卡因可抑制TPC-1细胞在裸鼠体内生长。结论利多卡因可以抑制甲状腺癌细胞TPC-1的增殖能力,其作用机制可能与抑制ERK信号通路激活有关。

关键词: 甲状腺癌, 利多卡因, 增殖

CLC Number:

R736.1

WU Yi, LIU Fu-ning, YAN Ying-zhe, YANG Hao-jie, XIE Qian. Lidocaine suppresses thyroid cancer cell TPC-1 proliferation via inhibiting activation of ERK signaling pathway[J]. Lingnan Modern Clinics In Surgery, 2021, 21(03): 294-297.

吴毅, 刘付宁, 严颖哲, 杨浩杰, 谢乾. 利多卡因抑制ERK信号通路激活降低甲状腺癌细胞TPC-1的增殖能力[J]. 岭南现代临床外科, 2021, 21(03): 294-297.

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