Porous ferroferric oxide targeted delivery of nigericin to induce pyroptosis combined with photothermal therapy for triple-negative breast cancer treatment

doi:10.3969/j.issn.1009-976X.2021.03.007

Abstract

Abstract: Objective To investigate the therapeutic effect of nigericin loaded with porous Fe₃O₄ nanoparticles combined with photothermal therapy for triple-negative breast cancer. Methods characterize synthesized porous Fe₃O₄ nanoparticles and loaded with nigericin. The Fe₃O₄@nigericin nanoparticle with 200 μg/ml was prepared to induce 4T1 pyroptosis. The cell killing ability was detected by CCK8, and the pyroptosis effect was verified by Western blot. Results The synthesized Fe₃O₄ showed uniform morphology and good photothermal conversion performance. The combination of loading nigericin and photothermal treatment showed stronger cell killing ability （P<0.001） and better pyroptosis effect （P<0.01）. Conclusion Porous Fe₃O₄ nanoparticles loaded with nigericin combining with photothermal therapy can produce very good therapeutic effect for triple-negative breast cancer by inducing pyroptosis, which is a potential new treatment method for triple-negative breast cancer.

Key words: porous Fe₃O₄ nanoparticles, pyroptosis, triple-negative breast cancer

摘要： 目的探讨多孔四氧化三铁（Fe₃O₄）纳米粒子负载尼日利亚菌素（nigericin）联合光热疗法用于三阴型乳腺癌的治疗效果。方法对合成的多孔Fe₃O₄纳米粒子进行表征,负载尼日利亚菌素,使用含有200 μg/mL的Fe₃O₄@nigericin诱导4T1细胞焦亡,通过CCK8检测细胞杀伤能力,western blot验证焦亡效果。结果合成的Fe₃O₄形貌均一,光热转化性能好,负载尼日利亚菌素联合光热治疗具有更强的细胞杀伤能力（P<0.001）,诱导的焦亡效果更好（P<0.001）。结论多孔Fe₃O₄纳米粒子负载尼日利亚菌素联合光热疗法可以通过诱导焦亡对三阴型乳腺癌产生非常好的治疗效果,是三阴型乳腺癌的一种潜在的新型治疗手段。

关键词: 多孔四氧化三铁纳米粒子, 焦亡, 三阴型乳腺癌

CLC Number:

R737.9

JIANG Wei, PAN Yue, YAO He-rui, HU Hai. Porous ferroferric oxide targeted delivery of nigericin to induce pyroptosis combined with photothermal therapy for triple-negative breast cancer treatment[J]. Lingnan Modern Clinics In Surgery, 2021, 21(03): 287-293.

江伟, 潘越, 姚和瑞, 胡海. 多孔四氧化三铁靶向递送尼日利亚菌素诱导细胞焦亡联合光热疗法用于三阴型乳腺癌治疗的研究[J]. 岭南现代临床外科, 2021, 21(03): 287-293.

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