Expression and significance of 8-Nitroguanine in intrahepatic cholangiocarcinoma with liver fluke infection

doi:10.3969/j.issn.1009-976X.2021.01.010

Abstract

Abstract: Objective To study expression and significance of 8-Nitroguanine in intrahepatic cholangiocarcinoma with liver fluke infection. Methods Expression of 8-nitroguanine in 18 intrahepatic cholangiocarcinoma tissue was detected by enzyme-linked immunosorbent assay （ELISA）, including paracancerous tissue and normal liver tissue. Serum levels of 8-nitroguanine in intrahepatic cholangiocarcinoma patients preoperatively and 18 clonochiasis sinensis infected patients and 18 healthy volunteers were measured by ELISA. RBE cells were treated with different concentrations of 8-nitroguanine and the 50% inhibiting concentration （IC₅₀） was determined by MTT assay. Invasive ability of RBE cell treated with 8-nitroguanine was detected by Transwell assay. Results In 18 intrahepatic cholangiocarcinoma, 8-Nitroguanine levels were significantly higher in tumor tissues than in peritumoral tissues and in normal liver tissues （P<0.05）, and there was no difference between in paracancerous tissues and in normal liver tissues（P>0.05）. And 8-Nitroguanine levels in serum, there was no difference comparable between in intrahepatic cholangiocarcinoma patients preoperatively and clonochiasis sinensis infected patients and 18 healthy volunteers（P>0.05）. 8-Nitroguanine suppressed the proliferation of RBE cells in a dose-dependent manner, but has no effect on cell invasion （P>0.05）. Conclusions Overexpression of 8-Nitroguanine in intrahepatic cholangiocarcinoma with liver fluke infection may be involved in the oncogenesis of disease, instead of tumor progression.

Key words: 8-Nitroguanine, intrahepatic cholangiocarcinoma, liver fluke infection, apoptosis

摘要： 目的探讨8-Nitroguanine在伴有肝吸虫感染的肝内胆管细胞癌（ICC）中的表达以及意义。方法应用酶联接免疫吸附试验（ELISA）检测2016年1月-2018年6月我院18例行根治性手术的伴肝吸虫感染的ICC患者癌组织、癌旁组织和正常肝组织中8-Nitroguanine的表达情况。同样用ELISA方法检测患者术前血清及18例肝吸虫性胆管炎和健康志愿者中8-Nitroguanine的表达情况;体外培养胆管癌RBE细胞,按梯度浓度处理细胞,计算半抑制浓度,并以该浓度处理细胞24 h,侵袭实验检测RBE细胞侵袭能力的变化。结果在18例伴有肝吸虫感染的ICC患者中,8-Nitroguanine在肿瘤组织中表达水平显著高于癌旁组织和正常肝组织（P < 0.001）,后两者差异无统计意义（P > 0.05）。伴有肝吸虫感染的ICC患者术前血清中8-Nitroguanine的表达水平与肝吸虫性胆管炎患者和健康志愿者无明显差异（P > 0.05）。8-Nitroguanine对RBE细胞的增殖能力具有浓度依赖性抑制作用,8-Nitroguanine处理后RBE细胞的侵袭能力无明显改变（P > 0.05）。结论 8-Nitroguanine与肝吸虫感染相关性ICC的关系密切,可能在癌变阶段发挥关键作用。

关键词: 8-硝基鸟嘌呤, 肝内胆管细胞癌, 肝吸虫感染, 细胞凋亡

CLC Number:

R735.7

LIN Jie, LIU Qing-bo, HE Wei, MA Jing, CHEN Yun-hao, WANG Wei-dong. Expression and significance of 8-Nitroguanine in intrahepatic cholangiocarcinoma with liver fluke infection[J]. Lingnan Modern Clinics In Surgery, 2021, 21(01): 59-62.

林杰, 刘清波, 何威, 马靖, 陈韵壕, 王卫东. 8-Nitroguanine在肝吸虫相关性肝内胆管癌中的表达以及意义[J]. 岭南现代临床外科, 2021, 21(01): 59-62.

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