Abstract: Objective To investigate the expression and clinical significance of long non-coding RNA（lncRNA）LINC00475 in renal cell carcinoma（RCC）, and analyze the effect of LINC00475 on the proliferation and migration of 786?O cells. Methods The expression of LINC00475 in RCC and adjacent normal tissues was detected by qRT-PCR. The clinical significance and prognostic value of LINC00475 in RCC was analyzed using TCGA data.LINC00475 expression was down?regulated by trans-fecting siRNA into 786-O cells. Later, the proliferation and migration of 786-O cells were detected by MTS and transwell assays. Finally, the potential molecular mechanism of LINC00475 involved in RCC was analyzed by gene enrichment analysis （GSEA）. Results The expression of LINC00475 in RCC tissues was significantly higher than in normal tissues. The expression of LINC00475 was positively asso-ciated with tumor stage（P=0.010）and M stage（P=0.022）. Meanwhile, patients with higher LINC00475 expression had a shorter survival time than patients with lower LINC00475 expression. Moreover, knock-down LINC00475 could significantly inhibit the proliferation and migration of 786-O cells. Finally, GSEA revealed that the gene sets associated with cyclin D1, VEGFA, WNT signaling pathway were signif?icantly up-regulated in LINC00475 high expression group（NOM P<0.01, FDR Q<0.05）. Conclusion LINC00475 was overexpressed in RCC and knockdown LINC00475 could inhibited the proliferation and migration of 786?O cells. LINC00475 might be an important biomarker in RCC.

Key words: LncRNA, clinical significance, renal cell carcinoma, LINC00475

摘要： 目的　探讨长链非编码RNALINC00475在肾癌中的表达情况及临床意义,并研究LINC00475对肾癌细胞786?O增殖、迁移能力的影响。方法　通过qPCR方法检测LINC00475在我院43对肾癌组织及癌旁正常组织中的表达水平。通过TCGA数据,分析LINC00475在肾癌中的表达水平，及其与肾癌患者临床指标、预后的关系。通过siRNA技术下调LINC00475，利用MTS法及Transwell法研究LINC00475对786-O增殖、迁移能力的影响。通过GSEA分析LINC00475在肾癌中可能参与的分子机制。结果　TCGA数据库肾癌组织及我院肾癌组织中,LINC00475在肾癌中的表达明显高于癌旁正常组织。TCGA数据分析表明，LINC00475的表达与肾癌患者的临床分期（P=0.010）、M分期（P=0.022）呈正相关。Kaplan?Meier生存分析表明,高表达LINC00475的肾癌患者总体生存时间明显低于低表达LINC00475的肾癌患者（Log?rankP=0.012）。下调LINC00475明显抑制786?O的增殖、迁移能力。GSEA分析结果　显示在LINC00475高表达组中,与cyclinD1、VEGFA、WNT信号通路相关的基因集明显上调（NOMP<0.01,FDRQ<0.05）。结论　LINC00475在肾癌组织中高表达,下调LINC00475可以抑制肾癌细胞786?O的增殖、迁移能力。

关键词: 临床意义, LINC00475, 长链非编码RNA, 肾癌