Abstract: Objective To explore the function and mechanism of circular RNA circ-VCAN in glioma. Methods The expression of circular RNA circ-VCAN in normal glial cells and glioma cells was confirmed by fluorescence quantitative PCR. Nucleocytoplasmic separationexperiments verified the distribution of circ- VCAN in the nuclear or cytoplasmic. RNaseR linear RNA digestion experiment was used to examine the circ- VCAN resistance to digestion of RNaseR. Circ- VCAN knockdown transfected cell lines were constructed. CCK8 was used to detect the cell proliferation ability after knockdown the circ- VCAN.Western blot was used to detect the expression levels of the NF- κB pathway proteins. Results When compared to the normal glial cells（HA1800）Circular RNA circ- VCAN was highly expressed in glioma cells，the differences were statistically significant（P<0.05）. Nucleocytoplasmic separation experiments demonstrated that circ-VCAN mainly existed in the cytoplasm. Circ-VCAN could resist digestion of RNaseR. Knock down circ- VCAN could inhibit the proliferation of U373 and T98G cells（P<0.05）. Western blot analysis showed that knockdown of circ-VCAN in U373 and T98G cells decreased expression of NF- κB pathway protein P- P65. Conclusion Knockdown circ- VCAN in the glioma cells can slow down the growth of glioma cells，suggesting that circ-VCAN may become a new therapeutic target.

Key words: circular RNA, glioma cell, proliferation

摘要： 目的 探索环状RNA-circ-VCAN在胶质瘤中功能及作用机制。方法 荧光定量PCR验证环状RNA-circ-VCAN在正常胶质细胞以及胶质瘤细胞中的表达情况；核质分离实验验证circ-VCAN在细胞核质分布情况；RNaseR消化线性RNA实验验证circ-VCAN对RNaseR消化的抵抗能力；构建敲低环状RNA-circ-VCAN的U373和T98G瞬时转染细胞株；CCK8实验检测敲低环状RNA-circ-VCAN后细胞的增殖能力；Westernblot检测NF-κB通路蛋白P-P65表达水平。结果 与正常胶质细胞（HA1800）相比，环状RNA-circ-VCAN在胶质瘤细胞（尤其是在U373和T98G）中高表达，差异均有统计学意义（P<0.05）；细胞核质分离实验证明circ-VCAN主要存在细胞质中；circ-VCAN能抵抗RNaseR的消化；CCK8实验证明敲低circ-VCAN能抑制U373和T98G的细胞增殖（P<0.05）；Westernblot检测敲低circ-VCAN后的U373和T98G细胞显示NF-κB通路蛋白P-P65表达水平减少。结论 在胶质瘤细胞中敲低circ-VCAN能减慢胶质瘤细胞的生长，提示circ-VCAN可能成为一个新的治疗靶点。

关键词: 环状 RNA, 胶质瘤, 细胞增殖