Abstract: Although the efficacy of surgery，chemotherapy and radiotherapy in colorectal cancer has been greatly improved in recent years ，15%-20% of patients with colorectal cancer had hepatic me? tastases when they were first diagnosed ，and 60% of the rest experienced metastatic or recurrent tumors. As a focus of clinical research in recent years ，circulating tumor cells have the tremendous potential in evaluating curative effect and prognosis. This article reviews the separation enrichment techniques ，detec? tion techniques and clinical significance of circulating tumor cells in colorectal cancer.

Key words: colorectal cancer, circulating tumor cells, clinical significance, separation enrichment techniques

摘要： 尽管近年来结直肠癌手术、化疗、放疗的疗效较前已有明显改善，但仍有15%~20%的结直肠癌患者在诊断时就已发现肝转移，而其余60%亦在后续的治疗过程中发现复发或转移。而外周血中循环肿瘤细胞（CTCs）的检测作为近年来临床研究的热点，在评估治疗癌症的疗效及判断预后等方面有着巨大的潜力。本文就CTCs的分离富集、检测技术及结直肠癌患者中CTCs检测的临床意义进行综述。

关键词: 结直肠癌, 分离富集, 临床意义, 循环肿瘤细胞