Abstract: 【Abstract】 Objective To investigate the function of EGFL7 in the research of cell invasion and migration in tongue squamous carcinoma. Methods EGFL7 and TSCC cell line expression levels were examined by qRT-PCR in cancer tissue and normal tissue. The changes about cell invasion and migration were detected by Transwell assays. Western blotting was performed to study the expression of EGFL7. Results After transfection with EGFL7-siR, the expression of EGFL7 was significantly down-regulated （P<0.001）. The invasion and migration ability of SCC-9 was substantially inhibited by EGFL7-siR. Western blotting showed that EGFL7 was found with positive expression in SCC-9. With downregulation of EGFL7，the expression of p-FAK was up-regulated substantially in SCC-9. Conclusion Overexpression of EGFL7 played an important role in the process of invasion and migration in SCC-9. Downregulation of EGFL7 in SCC-9 could substantially suppress its ability of cell invasion and migration. EGFL7 may exert its role by regulating the expression of p-FAK.

Key words: p-FAK, Invasion, Migration, Tongue squamous carcinoma, EGFL7

摘要： 【摘要】目的探讨EGFL7对舌鳞状细胞癌侵袭、转移能力的调控作用。方法通过qPCR验证舌鳞状细胞癌与癌旁正常组织以及舌癌细胞中EGFL7的表达水平差异，以舌鳞状细胞癌细胞株SCC-9为实验样品，通过脂质体介导，将EGFL7-siR转染至SCC-9细胞，降低EGFL7的表达水平。采用荧光实时定量RT-PCR检测转染前、后SCC-9中EGFL7的表达变化，通过Transwell实验检测细胞侵袭、迁移能力的改变，Western印迹检测EGFL7的下游信号分子FAK的表达变化。结果转染EGFL7-siR后的SCC-9中EGFL7的表达明显下调（P<0.001）。SCC-9细胞的侵袭迁移能力显著降低（P<0.001）。Western印迹检测结果显示，在舌鳞状细胞癌细胞株SCC-9中降低EGFL7的表达水平后FAK磷酸化水平降低，而FAK的表达水平不变。结论EGFL7的表达水平变化与舌鳞癌的侵袭转移能力密切相关；EGFL7可能通过调控其下游信号通路基因FAK磷酸化水平而发挥作用。

关键词: EGFL7, 舌鳞状细胞癌, 侵袭, 转移, p-FAK