Study of genistein on inducing apoptosis in prostate cancer DU-145 cells

doi:10.3969/j.issn.1009-976X.2016.02.023

Abstract

Abstract: 【Abstract】〓Objective〓To investigate the effects of genistein.(Gen).on the apoptosis of human prostate cancer DU-145 cells. Methods〓Human prostate cancer cell line DU-145 cells were treated with Gen with or without pan-caspase inhibitor Z-VAD(Z-VAD-FMK). Cell viability was examined by MTT assay..Apoptosis was determined using Annexin V-FITC/PI staining and flow cytometry..Mitochondrial membrane potential.(△ψm).was analyzed by JC-1 staining and flow cytometry. Caspase-8 activity was measured by colorimetric assay kits. Results〓Gen inhibited viability, induced apoptosis,.decreased △ψm and increased caspase-8 activity.(All P values were less than 0.05) in DU-145 cells. In addition, its effects could be reversed by Z-VAD. Conclusion〓Gen can induce apoptosis in human prostate cancer DU-145 cells by activation of both intrinsic and extrinsic apoptotic pathways.

Key words: Genistein, Prostate cancer, Apoptosis

摘要： 【摘要】〓目的〓探讨染料木黄酮诱导前列腺癌细胞系DU-145细胞凋亡的作用。方法〓在Caspase抑制剂Z-VAD存在或不存在的情况下,以染料木黄酮作用于前列腺癌DU-145细胞,采用MTT法检测细胞存活率、Annexin V-FITC/PI双染法流式细胞术检测细胞凋亡、PI染色法流式细胞术检测细胞周期、JC-1染色法流式细胞术测定细胞线粒体膜电位(△ψm),比色检测试剂盒测定caspase-8活性。结果〓染料木黄酮能明显降低前列腺癌DU-145细胞的存活率、诱导癌细胞凋亡、降低△ψm及提高caspase-8活性(全部P<0.05)。Z-VAD能够逆转Gen抗前列腺癌的效应。结论〓染料木黄酮使细胞阻滞于G0和G1,分别通过凋亡信号通路的外源性和内源性途径,诱导前列腺癌细胞凋亡。

关键词: 染料木黄酮, 前列腺癌, 凋亡

CLC Number:

R737.25

XIAO Congqin, HU Jianbo, ZHOU Yinan. Study of genistein on inducing apoptosis in prostate cancer DU-145 cells[J]. Lingnan Modern Clinics in Surgery, 2016, 16(02): 215-218.

萧聪勤胡建波周一南. 染料木黄酮诱导前列腺癌DU-145细胞凋亡的研究[J]. 岭南现代临床外科, 2016, 16(02): 215-218.

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