Abstract: 【Abstract】〓Objective〓To observe protein tyrosine phosphatase -3 (PRL-3) related regulatory function on epidermal growth factor (EGFR) signaling pathway in colon cancer LoVo cells. Methods After PRL-3 was transfected to LoVo cells (hereinafter referred to as LoVo-P) for 48 h, phosphorylation of EGFR protein expression and phosphorylation of MEK1/2 (p-MEK1/2), Erk1/2 (p-Erk1/2), Msk1/2 (p-Msk1/2) from MEK-ERK pathway were detected by Western blot assay. Results〓After transfected PRL-3, LoVo cells activated MEK-ERK channel. LoVo-C had an ability to activate phosphorylation of EGFR and Western-Blot showed an increase of 72.0% when compare with control group LoVo cells. High PRL-3 expression also induced to expression of p-MEK1/2, p-Erk1/2, p-Msk1/2. Conclusion〓In colon cancer cells, PRL-3 involved EGFR signaling pathway regulation by activation of phosphorylation of EGFR.

Key words: PRL-3, LoVo, EGFR, MEK-ERK

摘要：

【摘要】〓目的〓观察蛋白酪氨酸磷酸酶-3(PRL-3)在结肠癌LoVo细胞中对表皮生长因子(EGFR)相关信号通路的调节作用。方法〓转染PRL-3的LoVo细胞(以下简称LoVo-P)及对照组LoVo细胞(以下简称LoVo-C)48 h后,通过Western blot检测LoVo细胞PRL-3蛋白表达,并检测EGFR及相关信号MEK-ERK通路蛋白p-MEK1/2,p-Erk1/2,p-Msk1/2的表达。结果〓Western-Blot检测发现高表达PRL-3的LoVo细胞能够激活EGFR磷酸化水平,蛋白灰度分析显示其表达增多72.3%(P<0.01)。并且转染PRL-3后LoVo细胞的MEK-ERK通道被激活。结论〓PRL-3通过激活细胞表皮生长因子磷酸化参与表皮生长因子相关信号通路的调节。

关键词: PRL-3, LoVo, EGFR, MEK-ERK