Abstract: 【Abstract】〓Objective〓To explore the influence of microRNA-126 (miR-126) on proliferation and apoptosisof human hepatocellular carcinoma cell line SMMC-7721 and evaluate the correlation between miR-126 and PIK3R2. Methods〓After transfection of microRNA mimics into SMMC-7721 cells,cell proliferation and apoptosis were assessed by CCK-8 assay and cell apoptosis assay. Expression of PIK3R2 was evaluated by quantitative real-time polymerase chain reaction and western blotting. Luciferase reporter assay was used to confirm the relationship between miR-126 and PIK3R2. Results〓The outcomes by CCK-8 assay and apoptosis assay revealed that transfection of miR-126 suppressed cell proliferation, induced cell apoptosis of SMMC-7721 cells. Ectopic overexpression of miR-126 caused significant decrease of protein level of PIK3R2 (P=0.0135). In addition, luciferase assay showed that miR-126 significantly weakened the normalized PIK3R2-3’ UTR luciferase activity (P=0.0016). Conclusion〓Overexpressed miR-126 can inhibit proliferation and promote apoptosis of hepatocellular carcinoma cells by downregulating PIK3R2, thus indicating its tumor suppressive function in hepatocellular carcinoma.

Key words: microRNA-126, Hepatocellular carcinoma, Cell proliferation, Cell apoptosis, PIK3R2.

摘要： 【摘要】 目的 研究microRNA-126(miR-126)对肝癌细胞SMMC-7721迁移能力的影响以及对靶基因PIK3R2表达的影响。方法 通过慢病毒转染肝癌细胞株SMMC-7721使其过表达miR-126,利用CCK-8法检测细胞增殖能力。流式细胞术检测细胞凋亡;采用实时定量PCR和蛋白印迹检测细胞PIK3R2表达水平的变化,并通过荧光素酶实验验证miR-126与PIK3R2基因的直接调控关系。结果 与对照组相比,转染miR-126组的SMMC-7721细胞的增殖能力减弱,凋亡增加。过表达miR-126后,SMMC-7721细胞PIK3R2的蛋白表达下调(P=0.0135)。荧光素酶报告基因实验显示,miR-126能明显抑制PIK3R2-3’UTR的荧光素酶活性(P=0.0016)。结论〓过表达miR-126可能通过靶向降低PIK3R2基因的蛋白表达,抑制肝癌细胞的迁移。

关键词: 微小RNA126, 肝癌, 细胞增殖, 细胞凋亡, PIK3R2