Abstract: 【Abstract】〓Objective〓To evaluate the effect of Xuejie-Chitosan membrane on diabetic chronic wound of experimental rats and the expression of Wnt/β-catenin pathway. Methods〓Diabetic chronic wound model was made by intraperitoneal injection of streptozocin. The experimental group was treated with Xuejie-Chitosan membrane, and the control group was treated with conventional treatment. Western blot and qRT-PCR were used to determine the expression of β-catenin, VEGF and CyclinD1. Wound healing area was calculated by image-proplus 6.0. Results〓The wound healing rate in experimental group was significantly higher than in control group (P<0.01). Western blot and qRT-PCR results showed that both the mRNA and protein levels of β-catenin, VEGF and CyclinD1 were up-regulated when compared to the control group (P<0.01). Conclusion〓Xuejie-Chitosan membrane is superior to conventional treatment on chronic wound in diabetic rats. The activation of Wnt/β-catenin signals may help to promote healing of diabetic chronic wound.

Key words: Xuejie-Chitosan membrane, Wnt/β-catenin signal, Diabetic chronic wound

摘要： 【摘要】〓目的〓观察血竭壳聚糖药膜对糖尿病大鼠创伤愈合和创面组织Wnt/β-catenin信号通路的影响。方法〓采用链脲佐菌素(STZ)注射法建立大鼠糖尿病创面模型,实验组给与血竭壳聚糖药膜治疗,对照组采用常规换药治疗,观察两组创面愈合等情况。采用Western blot及qRT-PCR检测创面β-catenin、VEGF及CyclinD1 蛋白和mRNA的表达。结果〓血竭壳聚糖药膜能够明显促进糖尿病大鼠创伤的愈合,实验组创面愈合情况较对照组显著提高,差异具有统计学意义(P<0.01)。实验组创面组织β-catenin、VEGF及CyclinD1蛋白和mRNA的表达较对照组明显增加(P<0.01)。结论〓血竭壳聚糖药膜治疗大鼠糖尿病创面效果良好,其机制可能与活化Wnt/β-catenin信号有关。

关键词: 祛腐生肌, Wnt/β-catenin信号, 糖尿病创面