Abstract: 【Abstract】〓 Objective〓To investigate the expression and significance of CD4+CD25+ regulatory T cells for the patients with thromboangiitis obliterans (TAO). Methods〓Blood samples were collected from 32 patients with TAO, 35 patients with arteriosclerosis obliterans (ASO) (positive control) and 35 healthy people(normal control)..Treg,.transforming growth factor beta 1(TGF-beta 1)and interleukin 10(IL-10)..were detected by flow cytometry. Cytoplasmic antineutrophil cytoplasmic antibody (CANCA) and perinuclear anti neutrophil cytoplasmic antibody (PANCA) were determined by ELISA method. Results〓Compared with normal control group, Treg and TGF-beta 1 in TAO group were significantly increased(P<0.05),while those were significantly lower in ASO patients than that of normal control group. The level of IL-10 in TAO and ASO patients were lower than that in normal controls and there were no statistical difference between TAO and ASO patients. The expression of both CANCA and PANCA in TAO patients were increased significantly than that of normal control group, but there was no statistical difference between ASO patients and normal volunteers. Pearson correlation analysis showed that Treg and TGF-beta 1 and IL-10 either one with CANCA and PANCA had significant positive correlation (P<0.05). Conclusion〓Treg and TGF-beta 1 may be involved in the pathogenesis of TAO.

Key words: Thromboangiitis obliterans, Arteriosclerosis obliterans, Regulatory T cells, Anti neutrophil, cytokines

摘要： 【摘要】〓目的〓探讨CD4+CD25+调节性T细胞(Treg)与血栓闭塞性脉管炎(TAO)的关系。方法〓收集35例TAO患者,以35例动脉硬化性闭塞症(ASO)患者作为阳性对照组,35例健康人作为阴性对照组。采用流式细胞术检测外周血Treg及其转化生长因子β1(TGF-β1)及白介素10(IL-10)百分含量;采用ELISA法检测胞浆型抗中性粒细胞胞浆抗体(CANCA)和核周型抗中性粒细胞胞浆抗体(PANCA),并分析其相关性。结果〓与正常对照组比较,TAO组患者Treg及TGF-β1均显著升高(P<0.05),而ASO组患者Treg及TGF-β1则显著低于正常对照组,三组间Treg及TGF-β1两两差异具有统计学意义(P<0.05)。与Treg及TGF-β1不同,TAO及ASO组患者IL-10组间无统计学差异,且均显著低于正常对照组(P<0.0)。与正常对照组比较,TAO及ASO组患者CANCA及PANCA均呈不同程度升高,且TAO患者CANCA及PANCA均显著高于正常对照组,而ASO组患者与正常对照组之间并无统计学差异(P<0.05)。Pearson相关性分析显示Treg、TGF-β1及IL-10三者任意一者均与CANCA及PANCA呈显著正相关性(P<0.05)。结论〓Treg及TGF-β1升高可能参与TAO的发病机制。

关键词: 血栓闭塞性脉管炎, 动脉硬化性闭塞症, 调节性T细胞, 抗中性粒细胞, 细胞因子