Abstract:

【Abstract】〓Objective〓To study the effects of microRNA-155(miR-155) on migration and invasion of bladder cancer cell um-uc-3. Methods〓MiR-155 expression was detected in bladder cancer tissues. After exogenous transfection of miR-155 mimics into um-uc-3,.Transwell chamber assay and wound healing assay were performed to assess cell migration and invasion..Expression of EMT markers was evaluated by quantitative real-time PCR and western blotting. Results〓MiR-155 expression was higher in bladder cancer tissues than adjacent normal tissues...Over-expression of miR-155 promoted migration and invasion in bladder cancer cells of um-uc-3..Both mRNA and protein expressions of β-catenin, vimentin and snail were higher in miR-155 transfected cells, while claudin-1 showed no significance. Conclusion〓Over-expression of miR-155 can promote migration and invasion of bladder cancer cells with higher expressed EMT markers like β-catenin, vimentin and snail.

Key words: microRNAs, Bladder cancer, Migration, Invasion, EMT

摘要：

【摘要】〓目的〓前期研究发现miR-155在多种实体瘤中具有促肿瘤细胞增殖的作用,本研究探讨miR-155对人膀胱癌细胞um-uc-3转移相关的生物学行为的影响。方法 (1)通过组织RNA研究,来讨论miR-155在膀胱癌组织中的表达差异;(2)将人工合成miR-155-mimics转入人膀胱癌细胞um-uc-3,并设立对照组;(3)通过qRT-PCR检测miR-155的转染效率;(4)划痕试验研究过表达miR-155的um-uc-3细胞迁移能力变化;(5)Transwell试验检测miR-155对um-uc-3细胞迁移、侵袭的影响;(6)Western-blot及qPCR检测肿瘤转移相关基因的表达情况。结果〓(1)组织中,qRT-PCR证实miR-155的表达量在膀胱癌组织中较癌旁组织增高;(2)qRT-PCR检测miR-155转染效率,实验组表达量明显增高(P<0.01);(3)划痕实验证实miR-155过表达的um-uc-3细胞有更强的迁移能力;(4)Transwell试验发现miR-155可以促进um-uc-3细胞迁移、侵袭,与对照组差异显著(P<0.01);(5)过表达miR-155的um-uc-3细胞中,β-catenin、vimentin、snail的表达量在RNA水平和蛋白水平都比对照组显著增高,但不涉及claudin-1。结论〓miR-155可以促进人膀胱癌细胞um-uc-3的迁移和侵袭。

关键词: 膀胱癌, 微小RNA, 迁移, 侵袭, EMT