Abstract: 【Abstract】〓Objective〓To observe the changes and significance of natural killer T cells for the patients with prostate cancer undergoing extraperitoneal laparoscopic radical prostatectomy(ELRP). Methods〓Thirty-five patients with prostate cancer and 35 healthy subjects were enrolled. The peripheral blood of the two groups were collected, and the levels of NKT cells and its intracellular gamma interferon(IFN-γ) and interleukin 4 (IL-4) using flow cytometry test. Two weeks after ELRP, the prostate cancer patients underwent the second test of NKT and its cytokines. Results〓Compared with the healthy subjects,levels of NKT,IFN-γ and IL-4 belonging to the patients with prostate cancer were 5.92±1.47,.4.23±1.90 and 2.15±1.04, which were significantly lower than that of the healthy subjects (12.53±4.16, 8.94±2.76 and 5.86±1.97,.P<0.05). Levels of NKT, IFN-γ and IL-4 increased significantly after ELRP when compared with preoperation (9.13±2.69, 6.19±2.03 and 3.37±1.28, P<0.05),and the mean fluorescence intensity of IFN-γ increased more apparently than IL-4. Conclusion〓Decreasing of NKT, IFN-γ and IL-4 is an important immunological characteristic for patients with prostate cancer, and ELRP can improve the cellular immune dysfunction in patients with prostate cancer effectively.

Key words: Prostate cancer, Natural killer T cells, Cytokines, Immune regulation

摘要： 【摘要】〓目的〓通过自然杀伤T细胞(NKT)评价经腹膜外腹腔镜前列腺癌根治术(ELRP)的免疫调节效果。方法〓收集35例前列腺癌患者,以35例名健康对照组作为对照,采用流式细胞术检测外周血NKT及其细胞因子γ干扰素(IFN-γ)和白介素4(IL-4)含量。前列腺癌患者接受ELRP术后2周再次检测上述指标观察其变化。结果〓前列腺癌患者NKT百分含量、IFN-γ及IL-4的平均荧光强度分别为5.92±1.47、4.23±1.90及2.15±1.04,明显低于对照组的12.53±4.16、8.94±2.76及5.86±1.97(P<0.05);前列腺癌患者术后三者分别回升为9.13±2.69、6.19±2.03 及3.37±1.28,与术前差异有统计学意义(P<0.05),且均以IFN-γ的平均荧光强度改变较IL-4明显。结论〓NKT及其细胞因子数量下降是前列腺癌患者的一个重要免疫学特征,ELRP术可改善前列腺癌患者的细胞免疫功能紊乱。

关键词: 前列腺癌, 自然杀伤T细胞, 细胞因子, 免疫调节