Abstract:

【Abstract】〓Objective〓To explore the expression and significance of CD4⁺CD29⁺T cells for patients with gastric carcinoma. Methods〓Sixty-four patients with gastric cancer, including Ⅰ-Ⅳ stage, were enrolled, as well 60 chronic gastritis patients and healthy subjects. The peripheral blood CD4⁺CD29⁺T cells and cytokines γ-interferon (IFN-γ) and interleukin 4 (IL-4) were tested using flow cytometry. The levels of intercellular adhesion molecule 1 (ICAM-1), transforming growth factor beta 1 (TGF-beta 1) and carcinoembryonic antigen (CEA) were also tested. The correlation between the above-mentioned 6 indexes was analyzed via Pearson test. Results〓The percentage of CD4⁺CD29⁺T cells and mean fluorescence intensity of IL-4 of the gastric cancer patients were higher than those in the chronic gastritis and healthy subjects (P<0.05); whereas the mean fluorescence intensity of IFN-γ were lower than the other two groups for the gastric cancer patients. CD4⁺CD29⁺T cells and IL-4 increased and but IFN-γ decreased for the Ⅰto Ⅳ stage gastric cancer patients, and the levels of ICAM-1,.TGF-β1and CEA also increased when compared with the other two groups (P<0.05). Pearson analysis showed that CD4⁺CD29⁺T cells and IL-4 positively correlate with CAM-1, TGF-β1 and CEA; but IFN-γ negatively correlate with CAM-1, TGF-β1 and CEA (P<0.05). Conclusion〓Increase of CD4⁺CD29⁺T cells and IL-4,.as well the decrease of IFN-γ were the important immunological features for gastric cancer patients. CD4⁺CD29⁺T cells, IL-4 and IFN-γ were differently expressed in early and end stages for patients with gastric carcinoma significantly, and they correlated with the factors which are closely related the tumor angiogenesis.

Key words: Gastric carcinoma, CD4⁺CD29⁺T cells, Cytokines, Angiogenesis, Correlation analysis

摘要：

【摘要】〓目的〓探讨CD4⁺CD29⁺辅助性T细胞在胃癌患者中的表达及其临床意义。方法 收集Ⅰ~Ⅳ期胃癌患者共64例、慢性胃炎患者及健康对照组各60例作为观察对象。采用流式细胞术检测外周血CD4⁺CD29⁺T细胞及其细胞因子γ干扰素(IFN-γ)及白介素4(IL-4)水平;采用ELISA法检测三组的细胞间黏附分子-1(ICAM-1)、转化生长因子β1(TGF-β1)及癌胚抗原(CEA)的含量;分析以上六者的相关性。结果〓胃癌患者CD4⁺CD29⁺T细胞及IL-4水平显著高于慢性胃炎及健康对照组(P<0.05),而IFN-γ明显低于另外两组(P<0.05),但慢性胃炎与健康对照组上述三项指标无统计学差异。Ⅰ~Ⅳ期胃癌患者CD4⁺CD29⁺T细胞及IL-4呈递增趋势,而IFN-γ呈递减趋势,均表现为Ⅲ与Ⅰ期之间、Ⅳ与Ⅲ期之间均存在显著差异(P<0.05)。胃癌患者ICAM-1、TGF-β1及CEA显著高于慢性胃炎及健康对照组(P<0.05),但慢性胃炎与健康对照组上述三项指标无统计学差异。相关性分析显示CD4⁺CD29⁺T细胞及IL-4两者与ICAM-1、TGF-β1及CEA三者当中任意一者均呈正相关性,而IFN-γ与ICAM-1、TGF-β1及CEA三者当中任意一者均呈负相关性(P<0.05)。结论〓CD4⁺CD29⁺T细胞及IL-4表达增加、IFN-γ表达减少是胃癌患者的一个重要免疫学特征,在早晚期胃癌呈显著差异性表达,且三者与胃癌的血管表达相关因子密切相关。

关键词: 胃癌, CD4⁺CD29⁺T细胞, 细胞因子, 血管生成, 相关性分析