关键词: 髓鞘相关蛋白, 糖尿病模型小鼠, 链脲佐菌素, 热痛反应潜伏期

Abstract: Objective To observe correlation between thermal response latency and sciatic nerve myelin associated protein in streptozotocin?induced mouse with diabetes. Methods Sixteen 6?week?old male SPF grade C57BL/6J mice were randomly divided into two groups with 8 mice each. STZ 200mg/kg in STZ group was injected intraperitoneally once to establish the mouse model with diabetes. The same volume of buffer was injected in CON group. Mice were placed on a hot plate apparatus to value the thermal response latency. Real time RT qPCR and western blot were used to detect the expression of MAG，P0 in the sciatic nerve of mice. The correlation between the thermal response latency and MAG、 P0 was analyzed. Results Compared with CON group，the FBG level of STZ group was significantly increased（P<0.0001），which showed that the STZ induced diabetes model was successfully established. Compared with CON group，the thermal response latency of STZ group was longer（P<0.05）. The mRNA and protein expression levels of MAG、P0 were all significantly decreased in group A than those in group B（P<0.0001）. Conclusion The sciatic nerve myelin associated proteins are attenuated in the STZ?induced mouse model with diabetes. The results suggest that peripheral hyperglycemia may cause thermalgesia，which might relative to the decrease of myelin associated protein. But the specific mechanism re? mains to be explored.

Key words: Mouse diabetes model, Streptozotocin, Myelin associated protein, Thermal response latency