关键词: 脱细胞真皮基质, 脂肪干细胞, 扩张器

Abstract: Objective To investigate the effect of adipose?derived stem cells （ADSCs） on the expanded flaps. Methods Expander model was established by using the adult female SD rats. Between skin and the expander, theAcellular dermis matrix（ADM）to which ADSCs adhere is held under skin. The expanded skin flaps were harvested after three weeks. Immunohistochemically stainingwas performed to characterize the form of organization.Western blot was performed to explore the change of Col Ⅰ and fibronectin protein expression. Transforming growth factor-β 1 （TGF-β 1） was measured by QPCR. Results The histologic sections demonstrated that ADSCs promote TGF-β1. On the first week and the second week postoperatively, the ColⅠand fibronectin significantly increased with ADSCs, but reduced remarkably in the third week. QPCR demonstrated high expression of TGF-β 1 in the first week after operation, then it declined with time. Conclusion ADM loaded with ADSCs provided a new experimen-tal basis to promote reconstruction of dermis of expander flap in expander operation.

Key words: adipose?derived stem cells, expander, acellular dermis matrix