关键词: 肾脏纤维化, 钙黏蛋白6, TGF?β1, 单侧输尿管梗阻

Abstract: ［Abstract］ Objective To investigate the expression of cadherin6 （CDH6） in renal fibrosis. Methods Mice were subjected to unilateral ureteral obstruction（UUO）and kidneys were harvested at day 21 after operation. Hematoxylin and eosin （HE）staining was used for histopathologic examination. RT?qPCR was used to evaluate mRNA level of FSP?1, TGF?β1 and CDH6. Immunohistochemistry was used to measure protein level of FSP?1 and CDH6. HK?2 human renal proximal tubule epithelial cells were stimulated with TGF?β1. RT?qPCR or Western Blot was used to evaluate mRNA of FSP?1, TGF?β1, CDH6 or protein level of CDH6 respectively. Results Compared with Sham group, HE staining showed renal tubular atrophy and deposition of extracellular matrix in UUO group. mRNA levels of FSP?1, TGF?β1, CDH6 were up?regulated in obstructed kidneys while compared with sham?operated kidneys. Furthermore, immunohistochemistry showed that FSP?1 and CDH6 were lowly expressed in sham?operated kidneys while they were higher in obstructed ones. TGF?β1 facilitated the transition of HK?2 cells into morphology of fibroblast?like cells, up?regulating mRNA level of FN1, PAI?1, CDH6 and protein level of CDH6 in HK?2 cells. Conclusion Expression of cadherin6 and fibrosis?related biomarkers were upregulated in HK?2 cells induced by TGF?β1, accompanied with phenotype transition into mesenchymal cells. This may contribute to renal fibrosis induced by unilateral ureteral obstruction.

Key words: cadherin6, unilateral ureteral obstruction, renal fibrosis, TGF?β1