关键词: 巨噬细胞, 成纤维细胞, 感染, 伤口愈合

Abstract: Objective To investigate how the infection factors affect the activation and biologic function of dermal fibroblasts by regulating the activation phenotype of macrophages. Methods The activation phenotype of macrophages was assessed by flow cytometry ；the interaction between macrophages and fibroblasts was established by a transwell co- culture system ；the proliferation of dermal fibroblasts was determined by MTT assay；the expression of α-SMA in fibroblasts was detected by cell immunofluorescence；the secretion of type Ⅰ and type Ⅲ collagen was detected by ELISA；the mRNA expression of MMP9 and MM913 was detected by q-PCR. Results The M2 macrophages activated by IL-4 could significantly induced the expression of α-SMA，promoted the proliferation and secretion of type Ⅰ and type Ⅲ collagen of dermal fibroblasts. However，LPS could significantly induce macrophages activating to M1 phenotype and macrophages activated by LPS could dramatically inhibit the secretion of type Ⅰ and type Ⅲ collagen of dermal fibroblasts and promote the expression of MMP9 and MMP13. Conclusion Infection factors could affect the activation and biological function of dermal fibroblasts by regulating the activation phenotype of macrophages. We expected that people could improve wound healing by regulating the activated phenotype of macrophages in the wound area in the future.

Key words: macrophage, infection, fibroblast, wound healing