关键词: 结肠癌, 转化生长因子-beta, 肝再生磷酸酶-3, 侵袭

Abstract:

Objective To investigate role of TGF?beta in promoting the colorectal cancer LoVo cells invasion induced by phosphatase of regenerating liver-3（PRL?3）. Methods ELISA assay was to used to detect the level of TGF-beta in LoVo-P and LoVo-C. Invasion assays were applied to determine the effect of TGF-beta on the ability of PRL-3. Western blot was used to detect the proteins of p-AKT and AKT. Results Elisa assay displayed that the protein level of TGF?beta of LoVo?P was higher than LoVo-C （114 ± 11 pg/ml vs. 56 ± 8 pg/ml, P<0.01）. When added different dosages of neutralizing antibody of TGF-beta （1, 10, 00 ng/ml） in culture medium of LoVo-P, the invasion were decreased significantly（96.1 ± 8.2, 67.3 ± 9.4 and 48.6 ± 6.4, respectively）and all Pvaluesless than 0.05）. Western blot reminded the proteins of p?AKT in LoVo?P were higher than LoVo?C, and the expression of p?AKT in LoVo?P was decreased by 2.5?fold after treating LoVo?P with the PI3K inhibitor LY294002（10 mg/mL） （P<0.05）. Conclusion PRL?3 could up?regulate the expression of TGF?beta in colorectal cancer LoVo cells and promote the invasion of LoVo cells, in which PI3K/AKT signaling pathway may be involved.

Key words: PRL?3, invasion, colon cancer, TGF?beta