关键词: 皮肤成纤维细胞, 晚期糖基化终末产物, 长链非编码 RNA, 糖尿病皮肤病变, 糖尿病足

Abstract:

Objective   To explore the expression profile variation of long noncoding RNA （IncRNA） in advanced glycosylation end products （AGEs） treated dermal fibroblast compared with normal dermal fibroblast. Methods The differences of lncRNA expression profile between AGEs treated fibroblast and normal fibroblast were analyzed through lncRNA microarray. LncRNA with differential expression were screened out after pretreatment of raw data. Results Compared with normal fibroblast， 3421 lncRNAs were identified to be different expression （fold change≥2.0）in AGEs treated fibroblast. 1079 were up?regulated and 2342 were down?regulated. 448 lncRNAs （208 up?regulated and 240 down?regulated ） were highly differentially expressed with absolute fold change greater than ten. Conclusion      The expression profile of lncRNA in AGEs treated fibroblast was significantly changed in comparsion with normal fibroblast. These lncRNA may be involved in the occurrence and development of diabetic dermopathy. 

Key words: advanced glycosylation end products , diabetic foot, long noncoding RNA , dermal fibroblast , diabetic dermopathy