关键词: 实时定量RT-PCR, miR-20b, 乳腺癌

Abstract: 【Abstract】〓Objective〓To investigate the expressions of miR-20b in breast cancer and its role in breast cancer cells in vitro. Methods〓Total RNA from 134 breast cancer tissues were isolated for miR-20b quantitative analysis by real-time RT-PCR. The associations between miR-20b expression levels and clinicopathological features were analyzed. Breast cancer cells were transfected with miR-20b inhibitors,then CCK-8 and flow cytometry methods were used to detect the proliferation and apoptosis of the cells. Results〓The expression of miR-20b was up-regulated in breast cancer tissues compared with those in adjacent tissues (P<0.001). There was a significant correlation between miR-20b expression and tumor stage,distal metastasis and Ki67 expression (P<0.05). High miR-20b expression was positively correlated with shorter DFS and OS (P<0.05). The down-expression of miR-20b resulted in decrease in the rate of cell proliferation and increase in cell apoptosis. Conclusion〓Higher miR-20b is associated with poor prognosis of breast cancer and may serve as independent prognostic markers for breast cancer cells.

Key words: Breast cancer, miR-20b , RT-PCR