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岭南现代临床外科 ›› 2016, Vol. 16 ›› Issue (03): 344-347.DOI: 10.3969/j.issn.1009-976X.2016.03.027

• 论著与临床研究 • 上一篇    下一篇

提睾肌在无张力疝修补术后对大鼠生精功能的保护意义

王振龙 陈明 李称才 陈博艺 罗晓峰   

  • 通讯作者: 王振龙

The effect of cremaster-sparing hernia repair on spermatogenesis function in a rat model

WANG Zhenlong, CHEN Ming, LI Chencai, CHEN Boyi , LUO Xiaofeng   

  • Online:2016-06-20 Published:2016-06-20

摘要: 【摘要】 目的 探究提睾肌在无张力疝修补术后对大鼠生精功能的保护意义。方法〓选择成年SD大鼠建立实验动物模型,分正常对照组(NC组)、假手术组(Sham组)、手术组,手术组又分为OP1组和OP2组,前者保留提睾肌的完整性后者完全切断提睾肌。于术后90天分别将各组大鼠术侧睾丸组织HE染色观察病理变化,流式细胞仪测定各级生精细胞数量变化。结果〓OP1组生精过程轻度异常,生发上皮紊乱,生精细胞层数减少,可见大量的精子细胞和精子,各级生精细胞与Sham组相比差异无统计学意义。OP2组大鼠睾丸呈退行性变化,生发上皮紊乱,生精细胞层数减少,单倍体细胞及二倍体细胞数量减少,与OP1组及Sham组比较P<0.05。结论〓提睾肌对雄性大鼠的生精功能具有保护作用,男性腹股沟疝患者行无张力修补术无张力修补术时,应尽可能保护提睾肌的连续性和完整性。

关键词: 提睾肌, 无张力修补术, 生精功能,

Abstract: 【Abstract】 Objective〓To investigate the protective role of spermatogenesis by cremaster-sparing tension-free hernia repair in a rat model. Methods〓Twenty-four adult SD rats were randomized into 4 group, control group, sham group, operation group (OP1 and OP2, again). Op1 performed surgery by cremaster-sparing tension-free hernia repair, and cremaster was cut during operation in Op2 group. The animals were sacrificed in 90 days after experimental animal model establishment. The testiculartissue was taken for H.E staining and the assessment of quantity changes of spermatogeniccells in each group. The pathological changes of the testis in each group were observed. Results〓The testis of rats group OP2showed degenerative changes. The germinal epithelium disorder, the number of germinal cells were decreased, and the number of haploid cells and diploid cells were significantly decreased, as compared with group OP1 and group sham group (all P values were less than< 0.05). Conclusion〓Cremaster could be protective role on the spermatogenic function in rats underwent tension-free hernia repair. It is maybe an important step for hernia repair to protect the continuity and integrity of cremaster.

Key words: Hernia, Cremaster, Spermatogenesis, Tension-free hernioplasty

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