关键词: 胶质瘤, 树突状细胞, 氩氦冷冻治疗, 细胞因子

Abstract: 【Abstract】 Objective〓To explore the effect of combined argon-helium cryoablation and GM-CSF treatment on cytokine secretion of splenic DCs and CTLs cytotoxic activity in glioma-bearing mice...Methods〓Glioma-bearing mice models were established and divided into four groups of no-treatment,.GM-CSF injection,.cryoablation treatment,.and GM-CSF and cryoablation combined treatment. After seven days' treatment, the mice spleens were obtained, then the DCs were purified and cultured in vitro. The secretions of IL-10, IL-12p70 and IFN-γ were detected by ELISA. And the CD8+ CTLs were separated and cultured with GL261 cells in vitro..Then the cytotoxic activity of CD8+ CTLs were detected by LDH test. Results〓Combined treatment with cryoablation and GM-CSF could not only significantly decrease the secretions of IL-10, but also increase the secretions of IL-12 and IFN-γ..Furthermore,.combined treatment could signifcantly increase the cytotoxic activity of CD8+ CTLs..Conclusion〓Argon-helium cryoablation combined with GM-CSF treatment can further effectively activate T cells and enhance specific anti-tumor immunity through the stimulation of DCs and secretion of Th1 type cytokines.

Key words: Argon-helium cryoablation, Cytokine, Glioma, Dendritic cells